Sequences from confirmation amplicon


Confirmation sequences for line 179E06 and hit At1g74360 are shown.
Line ID 179E06
Locus At1g74360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27957073
Primers used for PCR JC21 - 8474, show primer details
   
Sequence Name 06-K103793-0022-179-E06C-JC21
GenBank Accession LN488781 [GenBank]
Sequencing primer gene specific primer, JC21: 5'-GCTCAGTGGAAATAAGTTTTCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K103793-0022-179-E06C-JC21
GCTGAGCACCTACATTTGGGTTTCATGAGTTTGAGGGGAAACTGCCTCCAGAGATAGGAC
AATTGCCTCTTGCATTCCTCAACCTTACTCGAAACAATTTCTCGGGCGAGATTCCTCAAG
AAATCGGAAATCTGAAGTGCCTGCAGAATCTTGATCTGTCTTTCAACAATTTCTCTGGAA
ACTTTCCAACGAGTTTGAATGACTTGAATGAGCTGAGTAAGTTCAACATCTCATATAACC
CTTTCATTTCTGGCGCGATACCAACAACAGGACAAGTAGCAACCTTTGACAAAGACTCCT
TTCTTGGAAATCCGCTGCTGCGGTTTCCTAGTTTCTTCAACCAATCAGGGAACAACACGA
GGAAGATTTCCAACCAAGTCCTAGGAAACAGGCCAAGAACTCTTCTTTTGATTTGGATTT
CCTTGGCTCTTGCATTGGCGTTTATTGCATGTTTAGTGGTATCAGGTATTGTACTTATGG
TTGTTAAGGCTTCAAGGGAAGCAGAAATCGATCTCTTAGATGGGTCGAAAACCAGACATG
ACATGACTTCCAGTTCAGGTGGATCATCGCCATGGCTATCAGGAAAAATCAAGGTCATTC
GCTTAGACAAATCAACTTTCACATACNCTGACATTCTGAAAGCAACCAGTAACCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTACAATTCAAAAATGTAAA
TGTCCCCACCGTTA
   
Sequence Name 14-K103794-0022-179-E06C-3144
GenBank Accession LN492479 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K103794-0022-179-E06C-3144
TTGAATATATCCTGGTTACTGGTTGCTTTCAGAATGTCAGCGTATGTGAAAGTTGATTTG
TCTAAGCGAATGACCTTGATTTTTCCTGATAGCCATGGCGATGATCCACCTGAACTGGAA
GTCATGTCATGTCTGGTTTTCGACCCATCTAAGAGATCGATTTCTGCTTCCCTTGAAGCC
TTAACAACCATAAGTACAATACCTGATACCACTAAACATGCAATAAACGCCAATGCAAGA
GCCAAGGAAATCCAAATCAAAAGAAGAGTTCTTGGCCTGTTTCCTAGGACTTGGTTGGAA
ATCTTCCTCGTGTTGTTCCCTGATTGGTTGAAGAAACTAGGAAACCGCAGCAGCGGATTT
CCAAGAAAGGAGTCTTTGTCAAAGGTTGCTACTTGTCCTGTTGTTGGTATCGCGCCAGAA
ATGAAAGGGTTATATGAGATGTTGAACTTACTCAGCTCATTCAAGTCATTCAAACTCGTT
GGAAAGTTTCCAGAGAAATTGTTGAAAGACAGATCAAGATTCTGCAGGCACTTCAGATTT
CCGATTTCTTGAGGAATCTCGCCCGAGAAATTGTTTCGAGTAAGGTTGAGGAATGCAAGA
GGCAATTGTCCTATCTCTGGAGGCAGTTTCCCCTCAAACTCATTGAAACCCAAATGTAGT
GTGCTCAGCCTGTCCATCTGAGAGATGCTTGCAGGAATCTCACCTGAAAACTTATTTCCA
CTGA
Primers used for PCR JC22 - 8474, show primer details
   
Sequence Name 07-K103793-0022-179-E06C-JC22
GenBank Accession LN489241 [GenBank]
Sequencing primer gene specific primer, JC22: 5'-CATGGTCAATACTCCGTAGCTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K103793-0022-179-E06C-JC22
ACTCGGGAGCTACATACCCGATTGTGCCTGCAATCACTGTGCTCACATGGCTATCTCCTA
CATTTAAGAGTCTTGCTAGTCCAAAATCCGTCACTCTCGCATTCCCGTGTTTATCCAAAA
GAACGTTACTGGCTTTGACGTCTCTGTGAACGATGGAAGGGTAGCATTCGTGGTGTAAAA
ACACTAATCCCCTTGCTACATCTGTGGCTATATCAATACGTTTCTTCCATTGAAGTTTTG
TTTTGTCTGTGATGAGCTCCTCTAAGCTCCCGCCTCCCATGTATTCGTGCACCAAGATTT
TCTCTGACCCATCTAGGCACCATCCATATAGCCTCACGAGGTTCGGATGTGCCCAATCTC
CAAATGCGTTGGCACTTAGAACTTCCATTTCAGCTCTGAACTCTTTCTCTGCTTCTGTGC
CTTCCCTCTGAAGCTTCTTGACTGCAACTTCTCTTCCATCAGGCAATACGCCTCTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAATATATAGATGTCCGCAGCGT
   
Sequence Name 15-K103794-0022-179-E06C-8409
GenBank Accession LN492942 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K103794-0022-179-E06C-8409
GCCATTTACGAGGCGTATTGCCTGATGGAAGAGAAGTTGCAGTCAAGAAGCTTCAGAGGG
AAGGCACAGAAGCAGAGAAAGAGTTCAGAGCTGAAATGGAAGTTCTAAGTGCCAACGCAT
TTGGAGATTGGGCACATCCGAACCTCGTGAGGCTATATGGATGGTGCCTAGATGGGTCAG
AGAAAATCTTGGTGCACGAATACATGGGAGGCGGGAGCTTAGAGGAGCTCATCACAGACA
AAACAAAACTTCAATGGAAGAAACGTATTGATATAGCCACAGATGTAGCAAGGGGATTAG
TGTTTTTACACCACGAATGCTACCCTTCCATCGTTCACAGAGACGTCAAAGCCAGTAACG
TTCTTTTGGATAAACACGGGAATGCGAGAGTGACGGATTTTGGACTAGCAAGACTCTTAA
ATGTAGGAGATAGCCATGTGAGCACAGTGATTGCAGGCACAATCGGGTATGTAGCTCCCG
AGTATGGACAAACTTGGCAAGCCACCACAAGAGGAGATGTTTACAGCTACNGAGTATTGA
CCATG

Last Updated on 06.12.2018 16:11