Sequences from confirmation amplicon


Confirmation sequences for line 941B09 and hit At1g74720 are shown.
Line ID 941B09
Locus At1g74720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28076178
Primers used for PCR KU83 - 8474, show primer details
   
Sequence Name 36-K103307-0022-941-B09C-8409
GenBank Accession LN502189 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K103307-0022-941-B09C-8409
TTACATTGAATATATCCTGTAAATATATCCACTGCGCATATCTCTTCGTTCGGATTGTGA
AGGCGCGTGGCTTACCACCTAACGAGAGCGCGTATGTTAAGGTACGGACGTCGAACCATT
TCGTCAGGTCTAAACCGGCCGTTAACCGGCCCGGCGAATCGGTTGATTCACCGGAGTGGA
ATCAGGTTTTTGCTCTTGGTCATAACCGGTCTGATTCCGCTGTAACTGGTGCGACTCTAG
AGATCTCTGCTTGGGATGCTTCGTCGGAGAGTTTTCTCGGAGGAGTTTGTTTTGATCTCT
CTGAGGTTCCGGTTCGTGACCCGCCGGATAGTCCGCTTGCTCCTCAGTGGTATCGGCTCG
AAGGCTCCGGCGCGGATCAAAACTCTGGGAGAATTTCCGGTGACATTCAGCTCTCTGTTT
GGATTGGTACTCAGGTAGATGAGGCATTTCCGGAGGCTTGGAGCTCTGATGCTCCGCAGG
TAGCTCACACGCGTTCTAAGGTGTATCANTCGCCCAAACTTTGGTACTTGAG
   
Sequence Name 12-K103308-0022-941-B09C-KU83
GenBank Accession LN491536 [GenBank]
Sequencing primer gene specific primer, KU83: 5'-ACCGTCACTCTCAAGTACCAAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K103308-0022-941-B09C-KU83
CGCGTGTGAGCTACATGCGGAGCACAGAGCTCCAAGCCTCCGGAAATGCCTCTATCTACC
TGAGTACCAATCCAAACAGAGAGCTGAATGTCACCGGAAATTCTCCCAGAGTTCTGATCC
GCGCCGGAGCCTTCGAGCCGATACCACTGAGGAGCAAGCGGACTATCCGGCGGGTCACGA
ACCGGAACCTCAGAGAGATCAAAACAAACTCCTCCGAGAAAACTCTCCGACGAAGCATCC
CAAGCAGAGATCTCAAGAGTCGCACCAGTTACAGCGGAATCAGACCGGTTATGACCAAGA
GCAAAAACCTGATTCCACTCCGGTGAATCAACCGATTCGCCGGGCCGGTTAACGGCCGGT
TTAGACCTGACGAAATGGTTCGACGTCCGTACCTTAACATACGCGCTCTCGTTAGGTGGT
AAGCCACGCGCCTTCACAATCCGAACGAAGAGATATGCGCAGTGGATATATTTACAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAG
A
Primers used for PCR KU84 - 8474, show primer details
   
Sequence Name 37-K103307-0022-941-B09C-OriL
GenBank Accession LN502581 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K103307-0022-941-B09C-OriL
GCCTTTACATTGAATATATCCTGTCCATTTACATGGCTTCATTCGTTCGTTCAACAATTG
AATATTCGTTAGGTGGTAAGCCATTTACAATTGAATATATCCGGCTCAACAAGATTGTAA
GGATGATGAGTCTTCTTCTCCATCGTCGTCTCTCCTCCTCCAGTTTTGCTATTGATAACC
CTAGGTGAATAATCTCCATTCGGTGGTCTCTTCGTAACCCTAATTCTATCTCCGCCGGGA
GGTCTTCCTACTTGCATCTTCCTCACTTCCGGTGGATAATAATGTACTTCCCCAGCCGAT
GGAGGTGGCGGCGGAGACGGTGGCCGTTGAGGATGGTTATCATTTCGGTGAGGATGGTTA
TCATTTGG
   
Sequence Name 13-K103308-0022-941-B09C-KU84
GenBank Accession LN492000 [GenBank]
Sequencing primer gene specific primer, KU84: 5'-TGTAATGCAAGGTCCAAATGATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K103308-0022-941-B09C-KU84
TACATCCTCACGGCCACCGTCTCCGCCGCCACCTCCATCGGCTGGGGAAGTACATTATTA
TCCACCGGAAGTGAGGAAGATGCAAGTAGGAAGACCTCCCGGCGGAGATAGAATTAGGGT
TACGAAGAGACCACCGAATGGAGATTATTCACCTAGGGTTATCAATAGCAAAACTGGAGG
AGGAGAGACGACGATGGAGAAGAAGACTCATCATCCTTACAATCTTGTTGAGCCGGATAT
ATTCAATTGTAAATGGCTTACCACCTAACGAATATTCAATTGTTGAACGAACGAATGAAG
CCNTGTAAATTGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATTTCCGCA

Last Updated on 06.12.2018 16:11