Sequences from confirmation amplicon


Confirmation sequences for line 874E06 and hit At1g75450 are shown.
Line ID 874E06
Locus At1g75450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28316321
Primers used for PCR 09af - 8474, show primer details
   
Sequence Name 09-K106611-0022-874-E06C-8409
GenBank Accession LN490192 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K106611-0022-874-E06C-8409
CCGGAATGAGCCATTTACAATTGAATATATCCGGACATGTAGATTGAATAGATTTCAGGA
AGTTGAGATTCTGATGAAGAAATTGAATTTCATACCGACATCGGTCTTTACAACGGATTT
ACAATATGTGGACTTTCTCGACCGGGTACACAAGGCCGAATTGAAGCTCCGGTCCAAGAA
TTTATGGGAGGTTCCACACCCATGGCTCAACCTCTTCGTGCCAAAATCAAGAATCTCTGA
CTTCGATAAAGGCGTTTTCAAGGGCATTTTGGGAAATAAAACAAGTGGCCCTATTCTTAT
CTACCCCATGAACAAAGACAAGTAAGTCTTGACATTACCATTGATTACTACTTCTAAATT
TCTTCTCTAGAAAAAAGAATAAAACGAGTTTTGCATTGCATGCATGCAAAGTTACACTTG
TGGGGATTAATTAGTGGTCCAAGAAAAAAAGTTTGTCAAAATTGAAAAAAACTAGACACG
TGGTACATGGGATTGTCCGAAAAACGTTGTCCACATGTGCATCGAACCAGCTAAGATTGA
CAACAACACTTC
   
Sequence Name 25-K106612-0022-874-E06C-09af
GenBank Accession LN497459 [GenBank]
Sequencing primer gene specific primer, 09af: 5'-CGAAGTGTTGTTGTCAATCTTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K106612-0022-874-E06C-09af
GTTTTCGGACATCCCATGTACCCGTGTCTAGTTTTTTTCAATTTTGACAAACTTTTTTTC
TTGGACCACTAATTAATCCCCACAAGTGTAACTTTGCATGCATGCAATGCAAAACTCGTT
TTATTCTTTTTTCTAGAGAAGAAATTTAGAAGTAGTAATCAATGGTAATGTCAAGACTTA
CTTGTCTTTGTTCATGGGGTAGATAAGAATAGGGCCACTTGTTTTATTTCCCAAAATGCC
CTTGAAAACGCCTTTATCGAAGTCAGAGATTCTTGATTTTGGCACGAAGAGGTTGAGCCA
TGGGTGTGGAACCTCCCATAAATTCTTGGACCGGAGCTTCAATTCGGCCTTGTGTACCCG
GTCGAGAAAGTCCACATATTGTAAATCCGTTGTAAAGACCGATGTCGGTATGAAATTCAA
TTTCTTCATCAGAATCTCAACTTCCTGAAATCTATTCAATCTACATGTCCGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAAAGTTATT

Last Updated on 06.12.2018 16:11