Sequences from confirmation amplicon


Confirmation sequences for line 013A04 and hit At1g75500 are shown.
Line ID 013A04
Locus At1g75500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28338409
Primers used for PCR 02yn - 8474, show primer details
   
Sequence Name 02-K122381-0022-013-A04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K122381-0022-013-A04C-8409
ATTGTCGTTTCACGCAAATTTCCTCTCTTCGCTCTTACCGTACAATACGAAGTAAAGTCC
CGCTATGATCAAGACCGCTCCAATAATCCTGCCATTAGAAATATGACATGTGGCACAACA
AATTTTAAAATTATTCTTGTTTAGGACCTTGGATTTTATCAAGTATTTATATAAACCACT
AATATTATTATAGTTTCATACCCGCCCAAATAAAATTCTTCGCCTAACGCAATAGAAGCC
ATGATCGCAACGACAAGAGTCTGAACAGGCTGGTAAACAGCAACGAAGACTGGACCCCCT
CTGTCAATACACCAAATCTGAACCGCAAACGCGATTCCAGACGCTACGATTCCCTGAAGT
TAAATCAATTCATTAGGACCAAATTGATTATTTTGAAACATTAGGGCCAAAAACGAATTA
ATTATAACAGATGAAAGAAAAACTAACGGCGTAGAGGATGGTGAAAAGCTCCCAACCGGA
GTGAAAAACCCAAGCCTGAGAATCTCTTTCACAGAAAGCAGCAATGATCAAGAACTGAAT
GATTCCGAAGAAACAAGTGTAAGACGTAACCGAGAGCCTCGCTGGATAAGACTTAAGAAC
CGGAGCTTGGAAAACAAGCCAGCCTGACCACGAGAGACAGTGACCGATTAGGTAGATGCA
ACCAAGGGTCCAGTTTTTAGGCGCGGCGTTTCCTAGCGGCGCTAAGACGGCGGAGTTTGT
GGTGAGTAGATGAGCGTGGAGGTGGCTAGCCGGAGTGTAGATGGTTGGACCTTTATAGAG
GGTGATGACGGAAGCTCCGGCGACACAAAGAGCTGTTCCTAGATTTTGGAGATACCGTCT
CTTCTGTTTATTCTTACTTTCTCAATCCTGAATTATGTTTTTTTCACATACAGTACATAA
ACAGTATGTTACATTGTTGTTACTACTATGATTATCACTATTTTGTAAGCAAAT
   
Sequence Name 26-K122382-0022-013-A04C-02yn
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 02yn: 5'-CTGCTCTTCTCAGGCACTTACTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K122382-0022-013-A04C-02yn
AATAACAATATTTTGGACAGCTTGGATATACAAGAACCACTTTAATAATGTCCATTTGAC
TTTTCAACGTAAAAGAAACGGATAATAATACACGTAGACAATATTTAATGCCGTTTCCAT
TTGCTTACAAAATAGTGATAATCATAGTAGTAACAACAATGTAACATACTGTTTATGTAC
TGTATGTGAAAAAAACAATAATTCAGGATTGAGAAAGTAAGAATAAACAGAAGAGACGGT
ATCTCCAAAATCTTAGGAACAGCTCTTTGTGTCGCCGGAGCTTCCGTCATCACCCTCTAT
AAAGGTCCAACCATCTACACTCCGGCTAGCCACCTCCACGCTCATCTACTCACCACAAAC
TCCGCCGTCTTAGCGCCGCTAGGAAACGCCGCGCCTAAAAACTGGACCCTTGGTTGCATC
TACCTAATCGGTCACTGTCTCTCGTGGTCAGGCTGGCTTGTTTTCCAAGCTCCGGTTCTT
AAGTCTTATCCAGCGAGGCTCTCGGTTACGTCTTACACTTGTTTCTTCGGAATCATTCAG
TTCTTGATCATTGCTGCTTTCTGTGAAAGAGATTCTCAGGCTTGGGTTTTTCACTCCGGT
TGGGAGCTTTTCACCATCCTCTACGCCGTTAGTTTTTCTTTCATCTGTTATAATTAATTC
GTTTTTGGCCCTAATGTTTCAAAATAATCAATTTGGTCCTAATGAATTGATTTAACTTCA
GGGAATCGTAGCGTCTGGAATCGCGTTTGCGGTTCAGATTTGGTGTATTGACAGAGGGGG
TCCAGTCTTCGTTGCTGTTTACCAGCCTGTTCAGACTCTTGTCGTTGCGATCATGGCTTC
TATTGCGTTAGGCGAAGAATTTTATTTGGGCGGGTATGAAACTATAATAATATTAGTGGT
TTATATAAATACTT

Last Updated on 06.12.2018 16:11