Sequences from confirmation amplicon


Confirmation sequences for line 588F08 and hit At1g75640 are shown.
Line ID 588F08
Locus At1g75640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28406324
Primers used for PCR EU10 - 8474, show primer details
   
Sequence Name 14-K103309x-0022-588-F08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K103309x-0022-588-F08C-8409
AAGCCATTTACAATTGAATATATCCTGGTTTTTTTATATAACTCAACCAAAAGGGTTGTA
ATTGGTTTTGTGCCTTCACACAAGATTTTGTGACAAAAGATGCTTACTTTGTCAAACCCT
GTTCATCGCTTATACACTTCAGCGTAACCGGTAATCACCTCACCGGTTTAATTCCGGTTA
CTTTAGGCACGATTCGAAGCCTGCAGGTGATTTCATTATCGGAGAATTCATTTACCGGTA
CAGTTCCGGTTAGTCTTCTATGTGGTTATTCAGGTTACAATTCCTCCATGCGAATCATTC
AATTAGGAGTTAATAACTTCACGGGAATAGCTAAGCCGTCAAATGCAGCTTGCGTGAACC
CTAATTTGGAGATTCTCGACATTCACGAGAATCGCATTAATGGCGATTTTCCCGCGTGGT
TGACTGATTTGACCTCTTTAGTGGTTTTGGATATTTCTGGAAACGGTTTCTCCGGTGGTG
TTACGGCTAAAGTTGGGAACTTGATGGCGCTTCAGGAGCTGAGAGTAGCTAACAATTCAC
TTGTTGGAGAAATTCCGACGAGTATCCGTAATTGCAAATCGTTAAGAGTCGTTGATTTTC
GAAGG
   
Sequence Name 06-K103310x-0022-588-F08C-EU10
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, EU10: 5'-CCTTCGAAATCAACGACTCTTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K103310x-0022-588-F08C-EU10
TCGGATTTCTCCACAAGTGAATTGTTAGCTACTCTCAGCTCCTGAAGCGCCATCAAGTTC
CCAACTTTAGCCGTAACACCACCGGAGAAACCGTTTCCAGAAATATCCAAAACCACTAAA
GAGGTCAAATCAGTCAACCACGCGGGAAAATCGCCATTAATGCGATTCTCGTGAATGTCG
AGAATCTCCAAATTAGGGTTCACGCAAGCTGCATTTGACGGCTTAGCTATTCCCGTGAAG
TTATTAACTCCTAATTGAATGATTCGCATGGAGGAATTGTAACCTGAATAACCACATAGA
AGACTAACCGGAACTGTACCGGTAAATGAATTCTCCGATAATGAAATCACCTGCAGGCTT
CGAATCGTGCCTAAAGTAACCGGAATTAAACCGGTGAGGTGATTACCGGTTACGCTGAAG
TGTATAAGCGATGAACAGGGTTTGACAAAGTAAGCATCTTTTGTCACAAAATCTTGTGTG
AAGGCACAAAACCAATTACAACCCTTTTGGTTGAGTTATATAAAAAAACAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
Primers used for PCR 07mg - 8474, show primer details
   
Sequence Name 28-K118466-0022-588-F08C-07mg
GenBank Accession LN499049 [GenBank]
Sequencing primer gene specific primer, 07mg: 5'-ATCCAAAACCACTAAAGAGGTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118466-0022-588-F08C-07mg
AAATTAGGGTTCACGCAAGCTGCATTTGACGGCTTAGCTATTCCCGTGAAGTTATTAACT
CCTAATTGAATGATTCGCATGGAGGAATTGTAACCTGAATAACCACATAGAAGACTAACC
GGAACTGTACCGGTAAATGAATTCTCCGATAATGAAATCACCTGCAGGCTTCGAATCGTG
CCTAAAGTAACCGGAATTAAACCGGTGAGGTGATTACCGGTTACGCTGAAGTGTATAAGC
GATGAACAGGGTTTGACAAAGTAAGCATCTTTTGTCACAAAATCTTGTGTGAAGGCACAA
AACCAATTACAACCCTTTTGGTTGAGTTATATAAAAAAACAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 01-K118465-0022-588-F08C-8409
GenBank Accession LN486783 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118465-0022-588-F08C-8409
ATTGAATATATCCTGTTTTTTTAATAACTCAACCAAAAGGGTTGTAATTGGTTTTGTGCC
TTCACACAAGATTTTGTGACAAAAGATGCTTACTTTGTCAAACCCTGTTCATCGCTTATA
CACTTCAGCGTAACCGGTAATCACCTCACCGGTTTAATTCCGGTTACTTTAGGCACGATT
CGAAGCCTGCAGGTGATTTCATTATCGGAGAATTCATTTACCGGTACAGTTCCGGTTAGT
CTTCTATGTGGTTATTCAGGTTACAATTCCTCCATGCGAATCATTCAATTAGGAGTTAAT
AACTTCACGGGAATAGCTAAGCCGTCAAATGCAGCTTGCGTGAACCCTAATTTGGAGATT
CTCGACATTCACGAGAATCGCATTAATGGCGATTTTCCCGCGTGGTTGACTGATTTGACC
TCTTTAGTGGTTTTGGAT

Last Updated on 06.12.2018 16:11