Sequences from confirmation amplicon


Confirmation sequences for line 069G06 and hit At1g75820 are shown.
Line ID 069G06
Locus At1g75820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28465717
Primers used for PCR 06ra - 3144, show primer details
   
Sequence Name 86-K115912-0022-069-G06C-06ra
GenBank Accession LN514689 [GenBank]
Sequencing primer gene specific primer, 06ra: 5'-TTGTTTGGTACAATCTCACCAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K115912-0022-069-G06C-06ra
AAGAGTTCTAACTTTCTCCTCAAGGTTTTGAATATCTCCAACAATGGTAACCTTACTGGA
ACATTCCCTGGAGAGATTTTAAAAGCTATGGTTGATCTTGAAGTTCTTGACACTTATAAC
AACAATTTCAACGGTAAGTTACCACCGGAGATGTCAGAGCTTAAGAAGCTTAAATACCTC
TCTTTCGGTGGAAATTTCTTCAGCGGAGAGATTCCAGAGAGTTATGGAGATATTCAAAGC
TTAGAGTATCTTGGTCTCAACGGAGCTGGACTCTCCGGTAAATCTCCGGCGTTTCTTTCC
CGCCTCAAGAACTTAAGAGAAATGTATATTGGCTACTACAACAGCTACACCGGTGGTGTT
CCACCGGAGTTCGGTGGTTTAACAAAGCTTGAGATCCTCGACATGGCGAGCTGTACACTC
ACCGGAGAGATTCCGACGAGTTTAAGTAACCTGAAACATCTACATACTCTGTTTCTTCAC
ATCAACAACTTAACCGGTCATATACCACCGGAGCTTTCCGGTTTAGTCAGCTTGAAATCT
CTCGATTTATCAATCAATCAGTTAACCGGAGAAATCCCTCANAGCTTCATCAATCTCAAA
CACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTC
TCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGT
GCGTCATCCCTTACGTCAGTGG
   
Sequence Name 69-K115911-0022-069-G06C-3144
GenBank Accession LN512267 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K115911-0022-069-G06C-3144
ATATAAGGGAAGTTTCATTTTCATTTGGAGTGGAACAGGGTACCCGGGGATCAGATTGTC
GTTTTCCCGCCTTCCGGTTTAAACTATCAGTGTTTGAGATTGATGAAGCTTTTGAGGGAT
TTCTCCGGTTAACTGATTGATTGATAAATCGAGAGATTTCAAGCTGACTAAACCGGAAAG
CTCCGGTGGTATATGACCGGTTAAGTTGTTGATGTGAAGAAACAGAGTATGTAGATGTTT
CAGGTTACTTAAACTCGTCGGAATCTCTCCGGTGAGTGTACAGCTCGCCATGTCGAGGAT
CTCAAGCTTTGTTAAACCACCGAACTCCGGTGGAACACCACCGGTGTAGCTGTTGTAGTA
GCCAATATACATTTCTCTTAAGTTCTTGAGGCGGGAAAGAAACGCCGGAGATTTACCGGA
GAGTCCAGCTCCGTTGAGACCAAGATACTCTAAGCTTTGAATATCTCCATAACTCTCTGG
AATCTCTCCGCTGAAGAAATTTCCACCGAAAGAGAGGTATTTAAGCTTCTTAAGCTCTGA
CATCTCCGGTGGTAACTTACCGTTGAAATTGTTGTTATAAGTGTCAAGAACTTCAAGATC
AACCATAGCTTTTAAAATCTCTCCAGGGAATGTTCCAGTAAGGTTACCATTGTTGGAGAT
ATTCAAAACCTTGAGAGAAGTTAGACTCTTCATCTCCAATGGTAATTCACCGGTGAAGTT
GTTGGCAGCTAAAGTTAGATTCACCAAATGAGTCAACATCCCAATCTCTGGT

Last Updated on 06.12.2018 16:11