Sequences from confirmation amplicon


Confirmation sequences for line 578A02 and hit At1g76030 are shown.
Line ID 578A02
Locus At1g76030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28536014
Primers used for PCR 32xr - 8474, show primer details
   
Sequence Name 76-K119544w-0022-578-A02C-32xr
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 32xr: 5'-TGAAGAGATGATACAGACAGGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K119544w-0022-578-A02C-32xr
AAGATTCCCTTTTCTCTGCTGCTGGTCTTCCACATAATGAAATAGCTGCTCAGATTTGTC
GTCAGGCTGGTCTAGTCAAGCGTTTGGAAAAGACTGTTGATCTACTTGAGGTAATCCTTT
GTAACTTGTACAAATATATCTCTTATTTCTCTATACCAAAGCTCTTATTTGAGTTGTAAC
TGTTTCTACAAGTGTGTTGATGGATGTGTGGTTACATAAAGGTCTTCATTATTAGATGTT
CTAGGCTTATAACTCAGTTGTTGTCAAAATTGAATTTCTTGTTTGTAGACATTCCTCTTC
AACGTTGGCTGTCTCATAATATCAAGGATTCTTTCTCATTCTTGTGTTTTTCCAGGATCA
TGGAGAGGACAATTTTGCAATTGTGTTTGCAGCTATGGGTGTGAACATGGAGACAGCTCA
GTTCTTCAAGCGAGATTTTGAAGAAAATGGATCAATGGAGAGAGTTACTCTTTTCCTGAA
CCTGGTAAATTTGACTTCTATAGCTGATTGCCATATCATGGAGTTTTTTCAAGTATTTAG
ATGTTTCTTAGCTTTAAACAGATTTAACCTTCCTTTATTCCAACAGGCCAATGACCCAAC
CATTGAGAGAATCATCACTCCTCGAATTGCCCTCACAACAGCTGAATATCTGGCTTATGA
ATGTGGGAAACACGTCCTTGTTATATTGACGGATATGAGTTCTTATGCTGATGCTCTTCG
TGAGGTAAAATGAATATTTGATACCGGAGTAGTTCCTTTATTTCATACCATGTTTTTTCC
TCTTCATGTTTGATAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATG
   
Sequence Name 48-K119543w-0022-578-A02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K119543w-0022-578-A02C-8409
TATCAAACATGAAGAGGAAAAAACATGGTATGAAATAAAGGAACTACTCCGGTATCAAAT
ATTCATTTTACCTCACGAAGAGCATCAGCATAAGAACTCATATCCGTCAATATAACAAGG
ACGTGTTTCCCACATTCATAAGCCAGATATTCAGCTGTTGTGAGGGCAATTCGAGGAGTG
ATGATTCTCTCAATGGTTGGGTCATTGGCCTGTTGGAATAAAGGAAGGTTAAATCTGTTT
AAAGCTAAGAAACATCTAAATACTTGAAAAAACTCCATGATATGGCAATCAGCTATAGAA
GTCAAATTTACCAGGTTCAGGAAAAGAGTAACTCTCTCCATTGATCCATTTTCTTCAAAA
TCTCGCTTGAAGAACTGAGCTGTCTCCATGTTCACACCCATAGCTGCAAACACAATTGCA
AAATTGTCCTCTCCATGATCCTGGAAAAACACAAGAATGAGAAAGAATCCTTGATATTAT
GAGACAGCCAACGTTGAAGAGGAATGTCTACAAACAAGAAATTCAATTTTGANCAACACT
GAGTTTATAGCCTAGAACATCTAATAATGAAGACCTTTATGTAAACACACATCCATCAAC
ACACTTG

Last Updated on 06.12.2018 16:11