Sequences from confirmation amplicon


Confirmation sequences for line 425A08 and hit At1g76810 are shown.
Line ID 425A08
Locus At1g76810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28833263
Primers used for PCR 09ey - 8474, show primer details
   
Sequence Name 60-K111269-0022-425-A08C-09ey
GenBank Accession LN510292 [GenBank]
Sequencing primer gene specific primer, 09ey: 5'-GTTGTGACAATAGGTCCCTGAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K111269-0022-425-A08C-09ey
AGTTCATGTCTACCCAGTGCCATCACTTGTTCGGGTCTGGATCATAAGTTTTTTACTTGC
CTGTAGCCCACAAACGACGATTTGATCACCTTCATGGAGTTCACCGTTTACCAACACAAC
ATCAATCGTTGTACCATGGCCTTCAATAACTTTGACCTCAAGTACAGTACACTGTTCAAC
AATTTCAAGAAAAGATCAGAAATCCGCATGCTGAAGTATTGCAGAAACAAGTGTTACCTA
AAGAGAAAGGAAAGAGACCTGCACTTCGTCAACATATGTAAGTTTCTCAACCATTGTTTT
CTGAGCCCATTGAACCAACCACAGCAAGAGATCTGGGACCCCTTCTCCATTGTAATATAC
TGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCACGNTTATT
   
Sequence Name 44-K111268-0022-425-A08C-8409
GenBank Accession LN505221 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K111268-0022-425-A08C-8409
TCATTGAATATATCCTGCAGTATATTACAATGGAGAAGGGGTCCCAGATCTCTTGCTGTG
GTTGGTTCAATGGGCTCAGAAAACAATGGTTGAGAAACTTACATATGTTGACGAAGTGCA
GGTCTCTTTCCTTTCTCTTTAGGTAACACTTGTTTCTGCAATACTTCAGCATGCGGATTT
CTGATCTTTTCTTGAAATTGTTGAACAGTGTACTGTACTTGAGGTCAAAGTTATTGAAGG
CCATGGTACAACGATTGATGTTGTGTTGGTAAACGGTGAACTCCATGAAGGTGATCAAAT
CGTCGTTTGTGGGCTACAGGCAAGTAAAAAACTTATGATCCAGACCCGAACAAGTGATGG
CACTGGGTAGACATAGAACTTTTCTAAGTGATCATATTTTCTTCCTCATATTTCAGGGAC
CTATTGTCACAA

Last Updated on 06.12.2018 16:11