Sequences from confirmation amplicon


Confirmation sequences for line 731C01 and hit At1g76850 are shown.
Line ID 731C01
Locus At1g76850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28848829
Primers used for PCR DN31 - 8474, show primer details
   
Sequence Name 38-K101830-0022-731-C01C-8409
GenBank Accession LN502924 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K101830-0022-731-C01C-8409
TTGAATAATCCTGATAACGAAACTAGTCAGAGCCACTTTCTTCCCAGGTGCAGGTGCTTT
ACCTTCTACTTTCTGAACCACGGGTGCTGTTCTCGATTCCCTCATATCACGAACCCTACG
AGCAAGCTGCCAAAATGCCACCGGAATTTCAGTGAAAATGGTCATCTTTCCACTTATACA
TCCATATGATGCTATGGTTCTAAAATCACTAATGGCATGCTAATTATCAAGCTCTCTATA
TCAACTCAACACCAGAAACTATGGATAGCATTCACCGTATCCCACGAATGGTATTGTTCA
GTGTCGTACAATTCAACAAATAGATCAATATATCGAATTCAAACTCTTGAACTTGATCGA
ACAAGCAAAGCCATAAGCATTCACTGATGAATCTCGCCACAAATACATACCTCAGCTTCA
TTAACCCGTTTCCAACAATCAGGCTCTCCTCCATCCCAAGCACCATCATCTTCCTTCCTC
GCTCTTCCCTTCTCTCGTACATCGCTACCTCTTCCTCTCCCCGCACCACCACTAGAACCA
CCTATT
   
Sequence Name 82-K101833-0022-731-C01C-DN31
GenBank Accession LN514172 [GenBank]
Sequencing primer gene specific primer, DN31: 5'-AGGGAAATAGGTGGTTCTAGTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K101833-0022-731-C01C-DN31
GGAGAGCGAGGAAGGAGATGATGGTGCTTGGGATGGAGGAGAGCCTGATTGTTGGAAACG
GGTTAATGAAGCTGAGGTATGTATTTGTGGCGAGATTCATCAGTGAATGCTTATGGCTTT
GCTTGTTCGATCAAGTTCAAGAGTTTGAATTCGATATATTGATCTATTTGTTGAATTGTA
CGACACTGAACAATACCATTCGTGGGATACGGTGAATGCTATCCATAGTTTCTGGTGTTG
AGTTGATATAGAGAGCTTGATAATTAGCATGCCATTAGTGATTTTAGAACCATAGCATCA
TATGGATGTATAAGTGGAAAGATGACCATTTTCACTGAAATTCCGGTGGCATTTTGGCAG
CTTGCTCGTAGGGTTCGTGATATGAGGGAATCGAGAACAGCACCCGTGGTTCAGAAAGTA
GAAGGTAAAGCACCTGCACCTGGGAAGAAAGTGGCTCTGACTAGTTTCGTTATCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGC

Last Updated on 06.12.2018 16:11