Sequences from confirmation amplicon


Confirmation sequences for line 199B11 and hit At1g77800 are shown.
Line ID 199B11
Locus At1g77800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29257720
Primers used for PCR 19nb - 8474, show primer details
   
Sequence Name 22-K114626-0022-199-B11C-19nb
GenBank Accession LN496220 [GenBank]
Sequencing primer gene specific primer, 19nb: 5'-TCCACAAAGAATTGTCAGAACTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K114626-0022-199-B11C-19nb
GGATTCTGATGTGGCTAGGATGACACCAATTTTGATGGTATAGACTTAATGATTCATGCC
ACAACTTTTAGGACATTTGGTAACATTAACTCATTTGGCAGGCTCTGAAGAAGGAAACAA
ACATCTGCAGGGTGCAGAGACATTTCTTCAGTTATCTAAAGCTAGGAAATTGGGCATACT
GGATCTGTCTCCTGAAGATGAATTGGAAGGAGAACTTCTATATTATCAGCTTCAGTTACT
TGGCACTGCAGTTTCAAGAAAGCAACTATCTGGTGATTTGATGATCCATTTTTAATTGCA
TGGTCCCGATTCTTCATGTTTTCTACACTATTTCTGTTATAGTATTCTAATAGTTGGTCC
TTAACAGACAATTTAGTCTATGAAGTTGCTAAAAAGCTGCCTCTGGAAATTGATGAACAA
CATGGACGAAGATGGGATGATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 22-K114625-0022-199-B11C-8409
GenBank Accession LN496219 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K114625-0022-199-B11C-8409
CATTTACATCATCCCATCTTCGTCCATGTTGTTCATCAATTTCCAGAGGCAGCTTTTTAG
CAACTTCATAGACTAAATTGTCTGTTAAGGACCAACTATTAGAATACTATAACAGAAATA
GTGTAGAAAACATGAAGAATCGGGACCATGCAATTAAAAATGGATCATCAAATCACCAGA
TAGTTGCTTTCTTGAAACTGCAGTGCCAAGTAACTGAAGCTGATAATATAGAAGTTCTCC
TTCCAATTCATCTTCAGGAGACAGATCCAGTATGCCCAATTTCCTAGCTTTAGATAACTG
AAGAAATGTCTCTGCACCCTGCAGATGTTTGTTTCCTTCTTCAGAGCCTGCCAAATGAGT
TAATGTTACCAAATGTCCTAAAAGTTGTGGCATGAATCATTAAGTCTATACCATCAAAAT
TGGTTGTCATCCTAGCCACATCAGAATCCGTGCTGCTTTTCAGGGTTTGTTCCTTGCCAA
GTTCTGACAATTCTTTGTGG

Last Updated on 06.12.2018 16:11