Sequences from confirmation amplicon


Confirmation sequences for line 884C09 and hit At1g78915 are shown.
Line ID 884C09
Locus At1g78915 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29671138
Primers used for PCR 48cs - 8474, show primer details
   
Sequence Name 31-K110172-0022-884-C09C-48cs
GenBank Accession LN500207 [GenBank]
Sequencing primer gene specific primer, 48cs: 5'-GACCAGATTTTCCTTCAAACTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K110172-0022-884-C09C-48cs
ACAATGCAGGAGTTTCTTGAGCAAACCTTTCAATTGAAACCAACTTTTTGAGATAAACTG
AAGAATGCAAAAATGAAGTTATGTTCGAGACACCATGAAGGAGAGCAGCAACATCTGTTA
CGTACAATATGCTTTAAAATCAAATTATACTTACTTCTGGGTACAGAAACAGACTGTTTA
GAAGATGATTTCCTGCAGCAAAGAAGTCCATATGAAAATATAACAACAGCAATTTAAAAT
TCTAAATGCTTTAAACAAACACAAAAACTATTTATACCTTTGCTGCAATGCCGGGTCTTT
CTCTTCCTGTGATGCGATTTCACATTTTTGTCTCAGTACTTTGAATCAACAAAATTAAGT
TGAACAAAGAAGAATTTGGAATCAAACCTTTTTTGAGCCGAAGCCACGCTTAGGGTTAGA
ATCCGAGCAACGTATTCGAAATAGGAGAAGCTTCGAGTTAAATGAAACGGAGGTCGTCGC
CGCTAGTGTCATCAACATCCTCTGTTTGTATTGTCCTAACCAAAACAGAGAATTTTCTAA
GAAAGTTTCAAAGAAATAATGAGAAGTAACGGCTACGAAGAGAAATTCGTCCGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCA
   
Sequence Name 15-K110171-0022-884-C09C-8409
GenBank Accession LN493019 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K110171-0022-884-C09C-8409
TTCTTTGAACTTTCTTAGAAAATTCTCTGTTTTGGTTAGGACAATACAAACAGAGGATGT
TGATGACACTAGCGGCGACGACCTCCGTTTCATTTAACTCGAAGCTTCTCCTATTTCGAA
TACGTTGCTCGGATTCTAACCCTAAGCGTGGCTTCGGCTCAAAAAAGGTTTGATTCCAAA
TTCTTCTTTGTTCAACTTAATTTTGTTGATTCAAAGTACTGAGACAAAAATGTGAAATCG
CATCACAGGAAGAGAAAGACCCGGCATTGCAGCAAAGGTATAAATAGTTTTTGTGTTTGT
TTAAAGCATTTAGAATTTTAAATTGCTGTTGTTATATTTTCATATGGACTTCTTTGCTGC
AGGAAATCATCTTCTAAACAGTCTGTTTCTGTACCCAGAAGTAAGTATAATTTGATTTTA
AAGCATATTGTACGTAACAGATGTTGCTGCTCTCCTTCATGGTGTCTCGAACATAACTTC
ATTTTTGCATTCTTCAGTTTATCTCAAAAAGTTGGTTTCAATTGAAAGGTTTGCTCAAGA
AACTCCTGCATTTGTTTGCAGAGGCACCTGGTCTGAATACTCAGTTTGAAGGAAAATCTG
GT

Last Updated on 06.12.2018 16:11