Sequences from confirmation amplicon


Confirmation sequences for line 499E11 and hit At2g01379 are shown.
Line ID 499E11
Locus At2g01379 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:171264
Primers used for PCR 22zb - 8474, show primer details
   
Sequence Name 19-K125592-0022-499-E11C-22zb
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 22zb: 5'-TATAATTTGACTTAGGACCTGGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K125592-0022-499-E11C-22zb
TTTTTGAGTGTAGAATCCATATATGAACCGAAACAGTTTCAGTTTGGTACGGGTTTGGTT
CTAGTTCTTTCGGTTATGAAGTTTGGGATCTATTCGGATATTTTATTTCAGATATTTCGG
ATATTTTCGAGTATTTTTTTGGATATTGTGGTGTTATATATATATTTTCAAGTATATTTT
GGTATTTTAGGTATTTTTGATTATTCAATCTAAAAATAACTAATATTTTTGGATAATATA
ATCATATTTCGGATATCTAAAATATTTTTGGGTTCGGTTCCTATTATTTTGGTTCTAGAA
TTTAGGATCTATTTAAGTATTTTACCATTTCGGTTCGGATTCGGGTTCGGTTCTTTTTGC
TTCGGTTCCGGTTCGGTTCTTTCGGTTCAGTTATTATGCCCACGCCTAATTTGACTTGTG
TTTTACTCGCTTATCTGTCCACTTTTATCTTCTTGTGAAATTTTCAAGTTACAAAAACAA
ATACTTGGCACCAAAATTGTGTGAATTAGTTAGTTAATGATCAAATGGTTAAATAACACA
AAGAGTTGTATAATTTATGTCCAAAAGGTATTACAATTTCGACTCTTTCGAGTTTGGAGG
AGTCGAATCGTGGATACATATATGTATGAGAAGAGAGACAATAAATAAAAGAATGTGGTT
GATCAAGCTTCAAGGGCAAGTAACATAAGTGTGGGAAGAATAAACTTGTGAATGAGAAAG
AGGGTTTAGTTTGTTGTGACATGGAAAAGGCTGTG
   
Sequence Name 03-K125591-0022-499-E11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K125591-0022-499-E11C-8409
CTTCTCATGTCACAACAAACTAAACCCTCTTTCTCATTCACAAGTTTATTCTTCCCACAC
TTATGTTACTTGCCCTTGAAGCTTGATCAACCACATTCTTTTATTTATTGTCTCTCTTCT
CATACATATATGTATCCACGATTCGACTCCTCCAAACTCGAAAGAGTCGAAATTGTAATA
CCTTTTGGACATAAATTATACAACTCTTTGTGTTATTTAACCATTTGATCATTAACTAAC
TAATTCACACAATTTTGGTGCCAAGTATTTGTTTTTGTAACTTGAAAATTTCACAAGAAG
ATAAAAGTGGACAGATAAGCGAGTAAAACACAAGTCAAATTAGGCGTGGGCATAATAACT
GAACCGAAAGAACCGAACCGGAACCGAAGCAAAAAGAACCGAACCCGAATCCGAACCGAA
ATGGTAAAATACTTAAATAGATCCTAAATTCTAGAACCAAAATAATAGGAACCGAACCCA
AAAATATTTTAGATATCCGAAATATGATTATATTATCCAAAAATATTAGTTATTTTTAGA
TTGAATAATCAAAAATACCTAAAATACCAAAATATACTTGAAAATATATATATAACACCA
CAATATCCAAAAAAATACTCGAAAATATCCGAAATATCTGAAATAAAATATCCGAATAGA
TCCCAAACTTCATAACCGAAAGAACTAGAACCAAACCCGTACCAAACTGAAACTGTTTCG
GTTCATATATGGATTCTACACTCAAAAACCGAAGGAATCGGAATCCAACTAAAACCGAAG
CGAATACCCGAACGCCCA

Last Updated on 06.12.2018 16:11