Sequences from confirmation amplicon


Confirmation sequences for line 296G09 and hit At2g01420 are shown.
Line ID 296G09
Locus At2g01420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:180899
Primers used for PCR KP03 - 8474, show primer details
   
Sequence Name 50-K021336-022-296-G09C-8409
GenBank Accession LN507036 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K021336-022-296-G09C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATAAATCTGACCGCCATGG
CAAACGTGGCGACAGAGTTCCCACAAGCAATGATTTTGGGTTGAAGTGCCATGAATAAAC
CTTAAAATTACACCAAAAATAAGCAATTCATTAACTAAAAGGATTTGAGAAAATTGACAG
CAAAGAGTTAGGTCATCTACTTGGTGAGACCCAAATAAGTAGACAGAGCCAGTGGGGACT
CGGCGTGGGGACCGGACACACCAACATGTCTGGGTCAGAGGGTCAGGATTCTCTCTTTGG
GTAAGTGCTTTGTACAGTTTATATGACTTTTACCAATTACAAGCTGGACAACTACGACTA
ATTAAACACACAGACCCACTTTGCATTAAATGAATCTTTTTTAACAGATAAGATGAGAAC
AAATTTGAACCCAACTTTAAAGATTAGATCATCATCAAAACAAGTCTAGATTTGCATGAA
GCAAGGTTAGTGTTTACCTAAGCTGAACATAGCCATTCCAAGACCAGCATCTGAGAGTAT
GGAGATGGATTGTTGTAATATTTTGGGCATAGCCACATGCCACCTGCTTCCAAAAACAAC
CCATACTCATTATTAGTTCCCACTACA
   
Sequence Name 51-K021336-022-296-G09C-KP03
GenBank Accession LN507366 [GenBank]
Sequencing primer gene specific primer, KP03: 5'-TATGCATGGAATAATTGTAGTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K021336-022-296-G09C-KP03
CTAATAATGAGTATGGGTTGTTTTTGGAAGCAGGTGGCATGTGGCTATGCCCAAAATATT
ACAACAATCCATCTCCATACTCTCAGATGCTGGTCTTGGAATGGCTATGTTCAGCTTAGG
TAAACACTAACCTTGCTTCATGCAAATCTAGACTTGTTTTGATGATGATCTAATCTTTAA
AGTTGGGTTCAAATTTGTTCTCATCTTATCTGTTAAAAAAGATTCATTTAATGCAAAGTG
GGTCTGTGTGTTTAATTAGTCGTAGTTGTCCAGCTTGTAATTGGTAAAAGTCATATAAAC
TGTACAAAGCACTTACCCAAAGAGAGAATCCTGACCCTCTGACCCAGACATGTTGGTGTG
TCCGGTCCCCACGCCGAGTCCCCACTGGCTCTGTCTACTTATTTGGGTCTCACCAAGTAG
ATGACCTAACTCTTTGCTGTCAATTTTCTCAAATCCTTTTAGTTAATGAATTGCTTATTT
TTGGTGTAATTTTAAGGTTTATTCATGGCACTTCAACCCAAAATCATTGCTTGTGGGAAC
TCTGTCGCCACGTTTGCCATGGCGGTCAGATTTATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAANNTATGGAGAANAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAA

Last Updated on 06.12.2018 16:11