Sequences from confirmation amplicon


Confirmation sequences for line 147B02 and hit At2g01760 are shown.
Line ID 147B02
Locus At2g01760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:333282
Primers used for PCR MI05 - 8474, show primer details
   
Sequence Name 53-K018335-022-147-B02C-MI05
GenBank Accession LN507995 [GenBank]
Sequencing primer gene specific primer, MI05: 5'-TCACTAACCGAAACAACAACAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K018335-022-147-B02C-MI05
TTCTTGTTATCCTCTAAAGCTTGAGAACTTCGAAGCTCTTTCTTCATCACACATTTCCTT
CTAACAACATGTTGCCAAATGTTCTTAAGCTCTTCAGGACGAATAGGTTTGATTAGATAA
TCACAAGCTCCATGGTTGATTCCAGTCATGACTGTAGTTGTTCTTCCATCAACAGACATC
ACTAGAAACAGAGCAAATGTAAGAAACATTGATGCAAAGATTCAATCTTTAGAGACAAAA
CATAACAACAGAGTCTTACTAATGACAGGAAGATCCATTTCAAGAAGACCAACTTGTTGA
AGAAGATTGTAACCATTCATACCAGGCATATGAACATCACTTAGCACCAAATCAAAACTG
TCTTTTCAATATTGTAAATGAACATCACTTAGCACCAAATCTTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTTTTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATTGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
   
Sequence Name 54-K018335-022-147-B02C-8409
GenBank Accession LN508330 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K018335-022-147-B02C-8409
TAGATTTCCCGGACATGAAGCCATTTACAAGATTTGGTGCTAAGTGATGTTCATTTACAA
TATTGAAAAGACAGTTTTGATTTGGTGCTAAGTGATGTTCATATGCCTGGTATGAATGGT
TACAATCTTCTTCAACAAGTTGGTCTTCTTGAAATGGATCTTCCTGTCATTAGTAAGACT
CTGTTGTTATGTTTTGTCTCTAAAGATTGAATCTTTGCATCAATGTTTCTTACATTTGCT
CTGTTTCTAGTGATGTCTGTTGATGGAAGAACAACTACAGTCATGACTGGAATCAACCAT
GGAGCTTGTGATTATCTAATCAAACCTATTCGTCCTGAAGAGCTTAAGAACATTTGGCAA
CATGTTGTTAGAAGGAAATGTGTGATGAAGAAAGAGCTTCGAAGTTCTCAAGCTTTAGAG
GATAACAAGAACAGTGGTAGCCTCGAGACTGTTGTTG

Last Updated on 06.12.2018 16:11