Sequences from confirmation amplicon


Confirmation sequences for line 458G01 and hit At2g02070 are shown.
Line ID 458G01
Locus At2g02070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:508026
Primers used for PCR 47ye - 8474, show primer details
   
Sequence Name 69-K120352-0022-458-G01C-47ye
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 47ye: 5'-ATTTATGTGTATGCGTGCAATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K120352-0022-458-G01C-47ye
AGGTGAATAAAATATGATGTATAATAGAGTAGTAGAGTGACTTTCTAGCCATCTATCTAC
TTTTCAAGATCCCATAATTACACGCGAATCTATTTGTAGCTACGTGACATGATGAGTCTC
TCTCTCTACTTACATTTATATTTTTTTTCTTTCACTTTTCCCAAAATATGTCTTTTATTT
CATCATTTTATATGAATAGTATAGAGATATATCTGACGATCCAATAGATTCCATTGTGAA
ATTGATTTTTATTTATTTTATTTTGTGTCACAGGCGAGACAGTTTCATCACACATAGAGC
TTTCTGTGACGCGTTGGCTCAAGAGAGTGCGAGACACCCAACTTCATTGACTTCTTTGCC
AAGTCATCACTTCTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTA
   
Sequence Name 66-K120351-0022-458-G01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K120351-0022-458-G01C-8409
CATTTACATTGAAATATCCTGCAGAAGTGATGACTTGGCAAAGAAGTCAATGAAGTTGGG
TGTCTCGCACTCTCTTGAGCCAACGCGTCACAGAAAGCTCTATGTGTGATGAAACTGTCT
CGCCTGTGACACAAAATAAAATAAATAAAAATCAATTTCACAATGGAATCTATTGGATCG
TCAGATATATCTCTATACTATTCATATAAAATGATGAAATAAAAGACATATTTTGGGAAA
AGTGAAAGAAAAAAAATATAAATGTAAGTAGAGAGAGAGACTCATCATGTCACGTAGCTA
CAAATAGATTCGCGTGTAATTATGGGATCTTGAAAAGTAGATAGATGGCTAGAAAGTCAC
TCTACTACTCTATTATACATCATATTATTATTCACCTGTGTAAACTCTTAACAAGCTTAC
ACACTCACATGTTTGATGCATACAATAAAATTGCACGCAACCACATAAA

Last Updated on 06.12.2018 16:11