Sequences from confirmation amplicon


Confirmation sequences for line 513C07 and hit At2g02148 are shown.
Line ID 513C07
Locus At2g02148 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:547621
Primers used for PCR 76ze - 8474, show primer details
   
Sequence Name 11-K124388-0022-513-C07C-76ze
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 76ze: 5'-AAATTAGGTTAACTACTTCCGGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K124388-0022-513-C07C-76ze
CCTGAAACCTAAACTTCTCGTAAAATATGTGCCTCAAAACGATATTTCAACCCGAGACTC
GAGCAAAGATTTGTCTCTCTTCACTATATCCCGAGTCTCTTGGAACCACACTCTGAGCAG
AATTTGGATGTCTCTCTCAAGTACTGTGCTCCACATTGGTCACAAAATTTCGCCGGACTA
TTTGGCTGTGGAAAGACAGATAAACATACGAGGACCTTAATGGAAATCGAAATTATACTA
TTAGAAAATAGATATAGCGTAGATTTTGTACCATTACATGGCCTCCAGTGAGGGGTTGCA
AGCAAGCCATTTGTGTAGAATTACGACTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTACACA
   
Sequence Name 01-K124387-0022-513-C07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K124387-0022-513-C07C-8409
CATTTACGTCGTAATTCTACACAAATGGCTTGCTTGCAACCCCTCACTGGAGGCCATGTA
ATGGTACAAAATCTACGCTATATCTATTTTCTAATAGTATAATTTCGATTTCCATTAAGG
TCCTCGTATGTTTATCTGTCTTTCCACAGCCAAATAGTCCGGCGAAATTTTGTGACCAAT
GTGGAGCACAGTACTTGAGAGAGACATCCAAATTCTGCTCAGAGTGTGGTTCCAAGAGAC
TCGGGATATAGTGAAGAGAGACAAATCTTTGCTCGAGTCTCGGGTTGAAATATCGTTTTG
AGGCACATATTTTACGAGAAGTTTAGTGTTTCAGGTTGGTGAAGTCTGAGCTAGTATATA
TTTACTTGGCCCGGAAGTAGTTAACCTAATT

Last Updated on 06.12.2018 16:11