Sequences from confirmation amplicon


Confirmation sequences for line 294C01 and hit At2g02820 are shown.
Line ID 294C01
Locus At2g02820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:805978
Primers used for PCR 13us - 8474, show primer details
   
Sequence Name 42-K117387-0022-294-C01C-13us
GenBank Accession LN504614 [GenBank]
Sequencing primer gene specific primer, 13us: 5'-CAATGAACTGAGAAGTTCAGCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117387-0022-294-C01C-13us
TTCTTAGAACACCTCTTGGTTACTTTTATCCTCACCTATTTAGCAGAAACACAACTTAAT
AGAATAAGACTCTCTATGTTTGCAACTTAATAGAAACACAACATGAAGAAGAATTTTGCG
CACCGTCACTCTCTTTCACATTGCCTATTTGTTTCTGCTCCTCTGTACCATCACTACTTG
TGGCATTGTGGGCTACTACAGAAAATGGATGTCTTCTCTGTTGATTCATAGTCGATTCAA
CCTTTATATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAACATTATTAT
   
Sequence Name 15-K117386-0022-294-C01C-8409
GenBank Accession LN493153 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117386-0022-294-C01C-8409
ATTTACATATAAAGGTTGAATCGACTATGAATCAACAGAGAAGACATCCATTTTCTGTAG
TAGCCCACAATGCCACAAGTAGTGATGGTACAGAGGAGCAGAAACAAATAGGCAATGTGA
AAGAGAGTGACGGTGCGCAAAATTCTTCTTCATGTTGTGTTTCTATTAAGTTGCAAACAT
AGAGAGTCTTATTCTATTAAGTTGTGTTTCTGCTAAATAGGTGAGGATAAAAGTAACCAA
GAGGTGTTTCTTAAGAAGGATGATTCAAAGGTGACAGCTTTGATGCAACAAGCTGAACTT
CTCAGTTCATT

Last Updated on 06.12.2018 16:11