Sequences from confirmation amplicon


Confirmation sequences for line 851B02 and hit At2g02860 are shown.
Line ID 851B02
Locus At2g02860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:831485
Primers used for PCR CV27 - 8474, show primer details
   
Sequence Name 03-K030598-022-851-B02C-8409
GenBank Accession LN487359 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K030598-022-851-B02C-8409
GCCAAAATAAACGAAGAAAAAGCACGCGATATTCCAAGGGTCTGCATCAACCAACCAAAT
GCAACACTGTTAGATAAAACTCAAAAGGGTTCTTCAGCAACAAGAAAATCAAAATACTTC
CAGCAAACAGATATTTAACAATAGTTCTATCTACCACCATAACTACCCACCTGATACATA
TATGACCAGTACAATACTATTCTAAACACAGTCTAATCTCTGATTATACAATAGTTCCGA
AAAAGGCTAAGGTCTTTTATATTTTTACAAATGCTTATGTAATCCAATGAACATAAACAA
TCTAGCTAAATTCCAAGGTCATATACTCTTCGATAAGCCAATGCTATAGAGTTCCATACG
AAAATATCTCA
   
Sequence Name 42-K030598-022-851-B02C-CV27
GenBank Accession LN504347 [GenBank]
Sequencing primer gene specific primer, CV27: 5'-GAGGGTTTTGTTCTTGTGTAGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K030598-022-851-B02C-CV27
CATAAGCATCTCGTAAAATCATAAAAGAACCTTAGCCTTTTTCCGGAAACTATTGTATAA
TCAGAGATTAGACTCGTGTTTAGAAATAGTATTCGTACTCGGTCATATATCGTATCAGGT
GGGTAGTTATGGTGGTAGATAGAACTATTGTTAAATATCTGTTTGCTGGAAGTATTTTGA
TTTTCTTGTTGCTGAAGAACCCTTTTGAGTTTTATCTAACAGTGTTGCATTTTGGTTGGT
TGATGCAGACCTTTGGATATCGCATGCTT

Last Updated on 06.12.2018 16:11