Sequences from confirmation amplicon


Confirmation sequences for line 742B09 and hit At2g03760 are shown.
Line ID 742B09
Locus At2g03760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1150080
Primers used for PCR KS54 - 8474, show primer details
   
Sequence Name 55-K101502-0022-742-B09C-8409
GenBank Accession LN508759 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K101502-0022-742-B09C-8409
AGATTCGATTTCTCCGTTTGCCTTCTCCAGACTGATGAACACGCACCTATCGCATCTTTC
GCTCCCCGAGTCTGTTAAGAGCTCGTCTTGTAAGATTGTGTATTGTTGTAGGAACCCTAA
GGACATGTTTGTGTCCTTATGGCATTTTGGGAAAAAGCTAGCTCCTGAGGAAACCGCGGA
TTATCCTATCGAAAAAGCGGTTGAAGCGTTTTGTGAAGGGAAGTTTATAGGTGGACCCTT
TTGGGATCATATATTGGAGTACTGGTATGCAAGCCGCGAGAATCCGAACAAGGTCTTGTT
TGTTACTTACGAGGAGCTAAAGAAGCAGA
   
Sequence Name 55-K101506-0022-742-B09C-KS54
GenBank Accession LN508760 [GenBank]
Sequencing primer gene specific primer, KS54: 5'-GTCTGCTTCTTTAGCTCCTCGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K101506-0022-742-B09C-KS54
CGGCTTGATACCAGTACTCCAATATATGATCCCAAAAGGGTCCACCTATAAACTTCCCTT
CACAAAACGCTTCAACCGCTTTTTCGATAGGATAATCCGCGGTTTCCTCAGGAGCTAGCT
TTTTCCCAAAATGCCATAAGGACACAAACATGTCCTTAGGGTTCCTACAACAATACACAA
TCTTACAAGACGAGCTCTTAACAGACTCGGGGAGCGAAAGATGCGATATGTGCGTGTTCA
TCAGTCTTGGAGAAGGCAAACTGGAGAAATCGAAATCTGGGGACTCGTAGTTTACTATAT
GGCCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11