Sequences from confirmation amplicon


Confirmation sequences for line 650H12 and hit At2g05420 are shown.
Line ID 650H12
Locus At2g05420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1984366
Primers used for PCR 15yp - 8474, show primer details
   
Sequence Name 48-K122428-0022-650-H12C-15yp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 15yp: 5'-TCTTTCTCTCTACCTCACTCAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K122428-0022-650-H12C-15yp
CCTTTTTTTATTCTCTTGACTATGTTCGTATATTCACAGTGGAATCTTAATACTTATATT
GGTTTGGTTTCTCTATATTGTTATTTGATTTTTGTTTTTTTTCTTCTGAAAGTCTTGGTG
AGTAGGAGTCATTATCAAAATCAATGGGGAAGCAAGTTGATAGTAAGACTATTACTTGGG
TGATTGAAAATTTCTCCTCTTTGCAATCCGCATCAATTCACTCTGATCAATTCGTGGTTG
GTGATTGCAAATGGTAAGAAAATAAATTGTTATCTAATGTTATCCCTAGAATATGGGTTT
AGCTGATTATTTTACTTTGGTTTTTGCTTGCAGGCGTCTTAAGGCCTATCCCAAGGGAAA
TGAAAAGGCGACTTATTTGGCTTATAGGGCTAATAATTTGGCTCTGTATTTAAACGTTGC
TAATAGTAAATCTTTCCCTATTGGATGGACAAGACACACTAAATTTTCTCTTACTTTAGT
AAATCAAAAATCAGAAAAACTCTCCAAACTAACAGGTTATTGTTCTCTATGCTTGCTCTA
TTTTATGGTTTATGTTATGTTGTAAACATTTATCTTAAAAGTTTTCTCTAACTTTAGTAA
ATCAATTTTAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGT
   
Sequence Name 24-K122427-0022-650-H12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K122427-0022-650-H12C-8409
TTGAATATATCCTAAAATTGATTTACTAAAGTTAGAGAAAACTTTTAAGATAAATGTTTA
CAACATAACATAAACCATAAAATAGAGCAAGCATAGAGAACAATAACCTGTTAGTTTGGA
GAGTTTTTCTGATTTTTGATTTACTAAAGTAAGAGAAAATTTAGTGTGTCTTGTCCATCC
AATAGGGAAAGATTTACTATTAGCAACGTTTAAATACAGAGCCAAATTATTAGCCCTATA
AGCCAAATAAGTCGCCTTTTCATTTCCCTTGGGATAGGCCTTAAGACGCCTGCAAGCAAA
AACCAAAGTAAAATAATCAGCTAAACCCATATTCTAGGGATAACATTAGATAACAATTTA
TTTTCTTACCATTTGCAATCACCAACCACGAATTGATCAGAGTGAATTGATGCGGATTGC
AAAGAGGAGAAATTTTCAATCACCCAAGTAATAGTCTTACTATCAACTTGCTTCCCCATT
GATTTTGATAATGACTCCTACTCACCAAGACTTTCAGAAGAAAAAAAACAAAAATCAAAT
AACAATATAGAGAAACCAAACCAATATAAGTATTAAGATTCCACTGTGAATATACGAACA
TAGTCAAGAGAATAAAAAAAAGGGTAGAACTATCGAAGTAGTTTAACTACTTACCTCCCT
GAGTGAGGT

Last Updated on 06.12.2018 16:11