Sequences from confirmation amplicon


Confirmation sequences for line 141A10 and hit At2g05810 are shown.
Line ID 141A10
Locus At2g05810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:2215405
Primers used for PCR ES08 - 8474, show primer details
   
Sequence Name 51-K020358-022-141-A10C-8409
GenBank Accession LN507363 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K020358-022-141-A10C-8409
TGATCCATGTCGATTTCCCGGACATGAAGCCATTTACAATTGATTCATTGCTGATCCATC
GTCACAACCATCACCGGAAGCTCTTTATTATTCATCCTCTCATTCCTCGGATCCAACATT
TGCACCAATCGAATCACACTCTTCTCATCTCTCATCAACTCCTTCCGATTTGATCTAACG
GTCAACAACGACTTCGCCGCCTCCGTCGCCGCTTCTTGTAACCCCGCCGGTTTCGGCGAT
TCCATTAACCGAATCAACGAGCTTAAACAATCAGCGACGGCTCGTTTGTTACCGTCGCTG
ATTGTTAAATTCGATAAAAGAGAAGTTGAGATCTGTTGAAGGATTACGTTTCCGTGTTTT
ATCAATTCACCTAATCGGATTATGAACCTTGTTGATGAAGATAAAACCCTAGAAACTGTT
TCCATGGCAGAGATCTGAGAGAGAGCAAGCAAACAGTGTTCGATCGTATCGGGATTTGAT
GATTCTTGAACTAAATGAATCAGAATCTGTAATCCTCCTCTTTCTCTAACAATCAAATCT
CTGTAATACTCACCGGAAGATGAGATCAAGGAGATGAAATTCGCGGTTTTCTCTTGTACC
GATGATGAACCGGAGATTAAGAGTTGGATAAGAACCGGTATAGCT
   
Sequence Name 55-K020358-022-141-A10C-ES08
GenBank Accession LN508669 [GenBank]
Sequencing primer gene specific primer, ES08: 5'-TTAGCTGAAGAAGGAGCTATACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K020358-022-141-A10C-ES08
TTATCCAACTCTTAATCTCCGGTTCATCATCGGTACAAGAGAAAACCGCGAATTTCATCT
CCTTGATCTCATCTTCCGGTGAGTATTACAGAGATTTGATTGTTAGAGAAAGAGGAGGAT
TACAGATTCTGATTCATTTAGTTCAAGAATCATCAAATCCCGATACGATCGAACACTGTT
TGCTTGCTCTCTCTCAGATCTCTGCCATGGAAACAGTTTCTAGGGTTTTATCTTCATCAA
CAAGGTTCATAATCCGATTAGGTGAATTGATAAAACACGGAAACGTAATCCTTCAACAGA
TCTCAACTTCTCTTTTATCGAATTTAACAATCAGCGACGGTAACAAACGAGCCGTCGCTG
ATTGTTTAAGCTCGTTGATTCGGTTAATGGAATCGCCGAAACCGGCGGGGTTACAAGAAG
CGGCGACGGAGGCGGCGAAGTCGTTGTTGACCGTTAGATCAAATCGGAAGGAGTTGATGA
GAGATGAGAAGAGTGTGATTCGATTGGTGCAAATGTTGGATCCGAGGAATGAGAGGATGA
ATAATAAAGAGCTTCCGGTGATGGTTGTGACGATGGATCAGCAATGAATCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA

Last Updated on 06.12.2018 16:11