Sequences from confirmation amplicon


Confirmation sequences for line 120D11 and hit At2g16400 are shown.
Line ID 120D11
Locus At2g16400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7104144
Primers used for PCR 81yp - 8474, show primer details
   
Sequence Name 19-K121846-0022-120-D11C-81yp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 81yp: 5'-TACATCGAAAGACGACACGACTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K121846-0022-120-D11C-81yp
TCCACCTTCAACAACAAGAAACAAACAACATTTATGTTACCACGAGAATCATTAACAAGT
GTAGAAGATATGTTGTAAGGCGGATTACCTCGTCTAATATCGATAAAAGTTTCGACAACT
TGCTCTGCAATTCTTGCCTCTCTGCTTGAGGGATTTCAGCAGTATTCGTCTGAAGATTCT
TCTCTTTCACTTCATTAATCTTATCTCCCTCTGGCTGAAACTGCTTTAGAGCTTTCTTAA
CATTAACAGTTTCATCAAGAAGCTCTTGAGCAGCTTTGAGATACTTTGAGTTGTGAATGG
TTCGAGTAAATCCCGAAACAACCTGTGTTGCATACTCATTGTTATTATTGCCTCTCGATG
TCTCAATCTGCGAGCTGAGGCCAAGACGCCTCATCACCGGGAAGCGATTCCTGTCATTGT
CTTCTTGATCTCTCCAAGAACCAGAATCTTGAATCTCAACCATTTGTGAAACTCCACCAA
GGCTTGACAACACTTGAATCTCTTTTGAAGGTGACACATTATTAGTTGAATCTCTAGGAA
ATGTCTCTGTGAAAGAATTTGTGTAGTTCATTGCGTTTCCAGGGACAAATTCTGGTCTGG
AATAAATCTCACTTGAGCCAGTTTTGTAATAAGTGGCCATAGTCTTCTTCTTCTTATGAT
CAGATCANACAAGGAGTCATCTGTTTCAGAATCAAACACAAACAACACTAAACCCTTTTT
ATACTTCACATTATGTAATAAAATCTTAATTAAGCAAATTTTAAGAAATTGAACCAATGA
AAATTATGAGAATTATCTCTCTGCATGAATTTAAGAATTTAANAAGAAGAAGAGGGGATA
GATTAAAGAATTACCAGGAAAGCATCTGCTGGCTTTGTGGGTGTTAAAGAGACACTAAAT
CTC
   
Sequence Name 03-K121845-0022-120-D11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K121845-0022-120-D11C-8409
TTGATTACAAATCGGACATGAAATATTTAAAAAATTGCATAGCACCAAAATTAGATATGA
AAAACGTTCATTATATATTTGTCTTATTCTCCAAATATGCTTGATAAACACATTATTATA
GAAGAATTACGAAAATATCTTTTACTATTGACACCAATCAGGAATATATCAAATATTTTT
AGATAAGAATATAAAAAACACAGAAAATGATTGTATGGTGGTTATAGCTTCATTCAATTT
TATTGCAGCAAACAAACAGAAAGGCCAGAGAGACGAAAGAGAGAGGCATTAGGGTTTAGG
ATTAGGTTTTAGAAAAGGGTTTAGATCTCTACCAACTGGTTTGTTAAGAACATAATCTCC
TTACCACATTTTTACTCTTGAAAACATTTTGATCTGTTTTTGTTATCTTCACCAAAAGAC
TTGACTCCAAGAAACAAGAAGAAGAATCAAGAAGCAAGTTCAAGAACCAGCTTTTAATGA
AATCTCTACTTCAAAAAGGAAACTTGCTTTAAGGAGGATGGATCTTTTGTTTTCTTGAAT
CTTTTGTGTGGGTAAAACATTTTGTATTATCAAGATTTTGATTTGAGGAGATAAAGAGTT
TGTTGTTAAGTTTGTGTTCTTGGGATCATCAAGAGTTAAAACAAAAAAAAAAAAACTTTT
TTTTTTCCCTCCTCCAATTGAAAATTAAGGGCCCCCTTAAACCCCCACAAACCCGGAAAA
GGTTTCCCGGGAAATTCTTAAACCTACCCTTTCTCCTTCTTTTAAAATCCTAAATTTCGG
GCAA

Last Updated on 06.12.2018 16:11