Sequences from confirmation amplicon


Confirmation sequences for line 285F01 and hit At2g18245 are shown.
Line ID 285F01
Locus At2g18245 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7938253
Primers used for PCR 06xs - 8474, show primer details
   
Sequence Name 04-K120096-0022-285-F01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K120096-0022-285-F01C-8409
CATTTACATTGAAAGAACCATTGAATAGCATTTAAAACTCTTGGTTACATGAAAGAGAGA
ACAAACCTTAGTATCAAGAGGATCTGCTCCTCCCGAGTCAATGATACAACCCTTAATCCT
TTCTACTAAATCTTGTCTACCAATGAAGCTCTCTAGCAAGGCACCATATCTACCAAAAGT
AAAAAAGCCCACCATGCTTAAAATTCATCATCTAATTCAAATCAACACTAAATCACAATC
GCAATCACAATGTAGGATTTTGAAGGATTTTGGTTTCATCAACCTACACAAGCCAACCAG
TGTTACTGAAACTGTGAAACACCAAGCATTTCTCTCTACCATCATCTTCTTTCTCCGATA
CCCAATTAACCAATTCATTCCCAAATTCAGCTATCCTTCGTTCAAGCCGCCGACCAAGAT
CTAACCGGAGCAAATCCCTAACGTCAACAGTGAAAGTAACGGCGTTAATTCCCCTGGAGT
TGTACCACTCAACGTATCTCCTTAGATGTTTAGCTTTCGCTCCTAGCCAACCAAGGAGAA
CCACCGTCGTCTCTTTCCCCTCGTTCCCGCCGAATATCGCCGCGTTTCCACCTCCGTTCA
CGATCCGTGGAGCTCTGTTCCAGACGGCGACGCCGTCGTTAGGTTCGGATCGGAATGGAT
TAGCGTGAACGGCGGTGTTCTCGAGACGAAACGTTGACGTTAGGAAGAAGAGTGATCTGA
AGAGATTCGCATTCGCAGGGAGCGAAGAGCACCGTATACGAGCGGCGGAGATAGAGGATT
TTCGGGTAACGGCATTGTGTAGTAGCTGATTTGTGGAATTGATATTACGGGGATCTGATT
TTAGATTGAGCTTACTGAGAAGAAGAAGACGATTGAGAGCAGAAGAAGTACTCAAGATTC
TTGTAGTTGCTTCCATGGC
   
Sequence Name 23-K120093-0022-285-F01C-06xs
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 06xs: 5'-ATACTCGGAGAACTGTGTGGACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K120093-0022-285-F01C-06xs
TTCTCAGCATTTATGTCTCTTGACTTCTATTGGTTTAGATATCGGTTTGGCAAATATTCA
TAGGGTTTATGCACAATTACATTTGCAACAACAACAAAATATAGTGAAGGATATAGCAAT
GAGAGATTACTGGAGAGACTCTATAAGACAGTTTCCGAGGAAGTGCAGTGCGTAAAACCG
CAGACTGTTGTCCAATTCAGATATGTTGTACTCTTTGATCCGAGAAAAAGATGGTGTAAA
CCAAAATGGAACATTTCAAAGTCTGAAAAGAGAGATTGAATCAGCTCCAAGGCATAAGGG
AAACCCGCTAATTCAGCAAGACTGTTTAATTTCCTATAAATATTCTATTTTTATTTTCTT
TTCAGATATTTATTCATCACTTATCAGCGATTTGACGAGAAGAGCTTTTTGGTTTCTGCG
AATCATCCAAAATCCGCCATGGAAGCAACTACAAGAATCTTGAGTACTTCTTCTGCTCTC
AATCGTCTTCTTCTTCTCAGTAAGCTCAATCTAAAATCAGATCCCCGTAATATCAATTCC
ACAAATCAGCTACTACACAATGCCGTTACCCGAAAATCCTCTATCTCCGCCGCTCGTATA
CGGTGCTCTTCGCTCCCTGCGAATGCGAATCTCTTCAGATCACTCTTCTTCCTAACGTCA
ACGTTTCGTCTCGAGAACACCGCCGTTCACGCTAATCCATTCCGATCCGAACCTAACGAC
GGCGTCGCCGTCTGGAACAGAGCTCCACGGATCGTGAACGGAGGTGGAAACGCGGCGATA
TTCGGCGGGAACGAGGGGAAAGAGACGACGGTGGTTCTCCTTGGTTGGCTAGGAGCGAAA
GCTAAACATCTNAGGAGATACGTTGAGTGGTACAACTCCGGGGGGAATTACGCCCGTACT
TTCACTGTTGACGTTAGGGATTTGCTCC

Last Updated on 06.12.2018 16:11