Sequences from confirmation amplicon


Confirmation sequences for line 206F07 and hit At2g18790 are shown.
Line ID 206F07
Locus At2g18790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8139810
Primers used for PCR 50xr - 8474, show primer details
   
Sequence Name 91-K119577-0022-206-F07C-50xr
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 50xr: 5'-CTAGTTATCGGAGACGAATTCTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K119577-0022-206-F07C-50xr
GATTTGACTTGAGGAGAAGAAATGGGGAGAATTGAGATCGGAGATAAGTGGAGAAACCAA
TAAAATAAATTGAAGAAGACGAAGCAAAATGTGTGCCTCTCAGATAGAGAGAGATAACAA
AAAAAAATCAGAGTTTTTTTTTTTTTGGCCCCCCCCCCTTTTGGTCCCCCCAAATTTTTC
CGGCCCTTTTTTTGGGGGGGGGTTTTTCCCAATTCCCCTTAAAGGTTTGGATTTTTCCCA
TTTACCCCCAAAAAACTTTTGGAAAGGGGTTCCGGGCCGGGAAACCCCCCGGGGTCCCCC
AGGGGTTTGGGGGGCCAATGGGGAAAAAAAAAAGGGGAATTCCCCTTTTTTTTTCCATTC
CAAAAGGGAAAGGCCCCCCCCCTTTTTAAAAA
   
Sequence Name 75-K119576-0022-206-F07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K119576-0022-206-F07C-8409
AAGCTCTTCTGGGTTAAATTGATAAATATCAAAACTTTAAAGGTAATTTGCTAAAATCGC
CACACAAAAAAAGTCGCAGAAAATATATGAGGAAACAAAAAGCGAAGACGACAAAAAAAA
AAAACCCCCGGTTTTTTTTTGGTTCCCCCCCCCTTCGGGAGGGGCCCCCTTTGGGCCCCC
CCTCCTCCATTTTTTTTTTGGGTTTCCCCCCTTTTCCCCCAACCCAATTCCCCCCCTTTT
CCTCCCCCCCCAGTTCAAAATTTCGGGAAATTTTCCCCCCCCAAAATTCCCCCCCCAAAA
CC

Last Updated on 06.12.2018 16:11