Sequences from confirmation amplicon


Confirmation sequences for line 324C12 and hit At2g18970 are shown.
Line ID 324C12
Locus At2g18970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8228825
Primers used for PCR 27ns - 8474, show primer details
   
Sequence Name 45-K114626-0022-324-C12C-27ns
GenBank Accession LN505619 [GenBank]
Sequencing primer gene specific primer, 27ns: 5'-AGGAGATTTTGAGTGAGATTGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K114626-0022-324-C12C-27ns
TTTAAATCTATTGTACAGATAATACTTCCTTGAAATAATATAATTCATTTGTTTCTTCTC
TTCAATATGATAAGGCGAATACAATCACAAAGCAAAAACTATAAATTTAAATTTGTTATA
TTGACGTCAAAAGAAATTTGTTGAAAGTTGAAATTTAGAAGCTGACTTTTGCCCAACAAT
ATATAATGTAATTATGTTAAACATGTAAATAAAGTGTGAAAGTCTGGTCAATAATTTTTA
TTTATTTGTTAATGTAAATGGCTTTGCAAAGTTTGGTATGGCTTCATTCACCTTTGAAGC
CATGTAGATTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCG
   
Sequence Name 45-K114625-0022-324-C12C-8409
GenBank Accession LN505618 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K114625-0022-324-C12C-8409
TTGAAATCTACATGGCTTCAAGGTGAATGAAGCCATACCAAACTTTGCAAAGCCATTTAC
ATTAACAAATAAATAAAAATTATTGACCAGACTTTCACACTTTATTTACATGTTTAACAT
AATTACATTATATATTGTTGGGCAAAAGTCAGCTTCTAAATTTCAACTTTCAACAAATTT
CTTTTGACGTCAATATAACAAATTTAAATTTATAGTTTTTGCTTTGTGATTGTATTCGCC
TTATCATATTGAAGAGAAGAAACAATGAATTATATTATATTCAAGAAGTATTATCTTGTA
CAATAGATTTTAAAACAATCATCTGCAATTCAAGACATACAATGTAGCTTCCAATCTCAC
TCAAAATCTCC

Last Updated on 06.12.2018 16:11