Sequences from confirmation amplicon


Confirmation sequences for line 824H04 and hit At2g19920 are shown.
Line ID 824H04
Locus At2g19920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8602380
Primers used for PCR NA26 - 8474, show primer details
   
Sequence Name 85-K101673-0022-824-H04C-8409
GenBank Accession LN514523 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K101673-0022-824-H04C-8409
GAGCCATTTACAATTGAATATATCCTGTAAATTGGTTTTTTGTTGATTGTAATGCTTTAT
AAACAAATCATTGGTTGAACGGTTTCTTCTCTCTGTAACAGGAGTTCTACAGTGCTGCAG
GGTTTAAAGAAAGCAAGAAAATTCTAGAAGAACTCTACCCAAAGGCCCTTGCACTTTACA
ACGTCACTTAGGACTATGCCATCAAGTGGAATAACGTTAAAAGTTGCGGGTTTGTCTGGA
AGGTTGCAGGTCCGGTCCTGTGCAGATTCTACGAGAAGAATTTAGTAATGTGTTCGATTT
CCATGCTGAAAGAGATTTTCGGCTGAACACTCTTACTGTTCTGTTCTGGTGATAACAATA
AGTATTTGCTGTCTTCATGTCTCATAATATGCGTGTTAGGCAAGTAACCTACTTATAGGC
CAAACCAAGATGTGTGGTGTAGTACTGGCTTTG
   
Sequence Name 85-K101680-0022-824-H04C-NA26
GenBank Accession LN514524 [GenBank]
Sequencing primer gene specific primer, NA26: 5'-CAAAGCCAGTACTACACCACACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K101680-0022-824-H04C-NA26
TTGCCTAAACGCATATTATGAGACATGAAGACAGCAAATACTTATTGTTATCACCAGAAC
AGAACAGTAAGAGTGTTCAGCCGAAAATCTCTTTCAGCATGGAAATCGAACACATTACTA
AATTCTTCTCGTAGAATCTGCACAGGACCGGACCTGCAACCTTCCAGACAAACCCGCAAC
TTTTAACGTTATTCCACTTGATGGCATAGTCCTAAGTGACGTTGTAAAGTGCAAGGGCCT
TTGGGTAGAGTTCTTCTAGAATTTTCTTGCTTTCTTTAAACCCTGCAGCACTGTAGAACT
CCTGTTACAGAGAGAAGAAACCGTTCAACCAATGATTTGTTTATAAAGCATTACAATCAA
CAAAAAACCAATTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT

Last Updated on 06.12.2018 16:11