Sequences from confirmation amplicon


Confirmation sequences for line 918D02 and hit At2g20050 are shown.
Line ID 918D02
Locus At2g20050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8651362
Primers used for PCR FQ83 - 8474, show primer details
   
Sequence Name 54-K033884-022-918-D02C-8409
GenBank Accession LN508393 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K033884-022-918-D02C-8409
AAGCCATTTCATTTCGAGTCAGCGGTCGGCCCTCTGACCAATATCCCAGAAGACGGTCCC
AAGTATGGCATTCTTCATGCTTGTATTTGAATATTTTTGTGGTTATCGTTGACTGATTCC
ATATAATGTTTTTTTTTCAGGACAAGACATTCTTCATTCGAGCTGTCAAAGGAATCTGCA
AAAGTTACCGATACCACAGCTCTTGCTAAAGCCACTCTTGCAGACCTGGTAAGCAATCTT
ACTCTACATCAATCTTAAAACGGTGCTCCTCAACTTACACTATTTTGGTTTATCCAGGAA
TGGACAACATGCTTGAGTACAACAGACTGTAGTGAGATTGGGCTCGTGCATTTGAAAGAT
AAAGAAAATTTGCTCAGCTTGAAAAGATTTTCAAAGCAAAAGGTGAAAAAGTTAGGTAAA
GAGGCACAAGTATTGAAAGAGCGGAATCTGATGAAGAACGTAATAAAGCCCTCAGCTATT
GTTCCCGAAATCTTGTGTACTTGTGTCGATCAAACATTTGCGGCAATCTTACTGAACACT
ACTCTTGCCTGTCCTATCTCTTCTCTGCTTCACTCTCCGCTTGACGAGTCATCTGTCCGT
TTCATTACCGGCTCACTTGTGTCTGCCATAGAAGACATACACAAGGTCAAAACTTTTTCT
TTAACATTTCTGGTTATTACTAAGATTTCGTGG
   
Sequence Name 14-K033884-022-918-D02C-FQ83
GenBank Accession LN492410 [GenBank]
Sequencing primer gene specific primer, FQ83: 5'-AAACAGAGTTTCAGACCACGAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K033884-022-918-D02C-FQ83
GTTAAAGAAAAAGTTTTGACCTTGTGTATGTCTTCTATGGCAGACACAAGTGAGCCGGTA
ATGAAACGGACAGATGACTCGTCAAGCGGAGAGTGAAGCAGAGAAGAGATAGGACAGGCA
AGAGTAGTGTTCAGTAAGATTGCCGCAAATGTTTGATCGACACAAGTACACAAGATTTCG
GGAACAATAGCTGAGGGCTTTATTACGTTCTTCATCAGATTCCGCTCTTTCAATACTTGT
GCCTCTTTACCTAACTTTTTCACCTTTTGCTTTGAAAATCTTTTCAAGCTGAGCAAATTT
TCTTTATCTTTCAAATGCACGAGCCCAATCTCACTACAGTCTGTTGTACTCAAGCATGTT
GTCCATTCCTGGATAAACCAAAATAGTGTAAGTTGAGGAGCACCGTTTTAAGATTGATGT
AGAGTAAGATTGCTTACCAGGTCTGCAAGAGTGGCTTTAGCAAGAGCTGTGGTATCGGTA
ACTTTTGCAGATTCCTTTGACAGCTCGAATGAAGAATGTCTTGTCCTGAAAAAAAAACAT
TATATGGAATCAGTCAACGATAACCACAAAAATAAACAAATACAAGCATGAAGAATGCCA
TACTTGGGACCGTCATCTGAAATATTGGTCAGAGGGCCGACCGCTGACTCGAAATGAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11