Sequences from confirmation amplicon


Confirmation sequences for line 364E07 and hit At2g20120 are shown.
Line ID 364E07
Locus At2g20120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8688265
Primers used for PCR GC52 - 8474, show primer details
   
Sequence Name 45-K033543-022-364-E07C-8409
GenBank Accession LN505435 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K033543-022-364-E07C-8409
CCATTGTTCGTCCAAACGGCATCCGCTTGATAAACCACTCTCCCAAGTTCAGAACCGATG
CCCCCAACCACGAAGACATGAACACCCCAACCAAGAAGATGAATGCAATGGAAGTCAAAA
AACCGAAACCTACAAAAAACCATAATCAAACTTATTGTGGGAAACGGAACGATATCAAAT
TGTTCATTCGATAAAGAAGGGCCACTCAAATTCTTACCAAATACGTTGATTCCAAGTTGT
GCATATATTGGAGAGAAGAATCCATCGACAAAGTGAATAAACCACCATGTTATATAGAAA
GTAATTGCAATAGGAAGTAGGATAACACTACAAAAAGCAATTGAGATTTGAAACTATCAG
CACAAATCTTGAATATGTGACAAGTTTCATCAAGCCAAAATTTCAGTAAACTCTTCCTTA
CCAGCCTGTCATGAACTTCTTTGACGCCCAGCCCCTAATGAACAAGCTTAAAGTCTGCAG
AAAAATAGATAAAGCATCAGATCACAATGCTCCTATGTGTTATATATGCTTGAAAACATG
AATTAGCATG
   
Sequence Name 45-K033543-022-364-E07C-GC52
GenBank Accession LN505436 [GenBank]
Sequencing primer gene specific primer, GC52: 5'-TCATGCTAATTCATGTTTTCAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K033543-022-364-E07C-GC52
TGATGCTTATCTATTTTTCTGCAGACTTTAAGCTTGTTCATTAGGGGCTGGGCGTCAAAG
AAGTTCATGACAGGCTGGTAAGGAAGAGTTTACTGAAATTTTGGCTTGATGAAACTTGTC
ACATATTCAAGATTTGTGCTGATAGTTTCAAATCTCAATTGCTTTTTGTAGTGTTATCCT
ACTTCCTATTGCAATTACTTTCTATATAACATGGTGGTTTATTCACTTTGTCGATGGATT
CTTCTCTCCAATATATGCACAACTTGGAATCAACGTATTTGGTAAGAATTTGAGTGGCCC
TTCTTTATCGAATGAACAATTTGATATCGTTCCGTTTCCCACAATAAGTTTGATTATGGT
TTTTTGTAGGTTTCGGTTTTTTGACTTCCATTGCATTCATCTTCTTGGTTGGGGTGTTCA
TGTCTTCGTGGTTGGGGGCATCGGTTCTGAACTTGGGAGAGTGGTTTATCAAGCGGATGC
CGTTTGTGACGAACAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATT

Last Updated on 06.12.2018 16:11