Sequences from confirmation amplicon


Confirmation sequences for line 348G10 and hit At2g20380 are shown.
Line ID 348G10
Locus At2g20380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8795386
Primers used for PCR 08vy - 8474, show primer details
   
Sequence Name 26-K118578-0022-348-G10C-08vy
GenBank Accession LN498140 [GenBank]
Sequencing primer gene specific primer, 08vy: 5'-GTCAAACAGTAGCAGATTTAGCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118578-0022-348-G10C-08vy
GGCATCGAAACCACTCGAATTCTTTCCCCAAATTCTCTTTTCTGGCCGCGCTTTTTCAAG
GGAAATTTCTGCGCACCAAACCATCTTCTCCTCATACCCACTTGCAGGCACATACTCGTG
CCACAAAACAACCAGCTTACCACCACAATCCGCCAACTTAACACTACTATATCTAGCAAA
CTTAGGTAGCCCTTCTAATCCCACTAATGATCTCCAAACTCCTCCCTGTCTTCTTCTCCA
TCTAAACTTTACACTGGGGTCATAATGATAGGTAATCTCCTCTATCATGCAAAAAGAACC
TCGACCTAAATCTGTATTGACCGAAAATCCTAACACTTCATATCCTTCCGAAACCTTTGG
CTTAAAAGCCACAACCTCCGTAGAAAGTAATGACCGAAGCTTCGGAAATAAAGGCGCAAG
CTCCTCAGCATCCTGTGCAACAAAGCTCAAAAGTTTACTAACTTTCATGTAAAGCATTCC
TTTCATATGTTGGAGTTTGAATATCCGTTTGTTGGGCGGCACAGGAATCCAAGTTTGAGT
GTCTTTCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TT
   
Sequence Name 10-K118577-0022-348-G10C-8409
GenBank Accession LN490876 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118577-0022-348-G10C-8409
CGCCCAACAAACGGATATTCAAACTCCAACATATGAAAGGAAAGCTTTACATGAAAGTTA
GTAAACTTTTGAGCTTTGTTGCACAGGATGCTGAGGAGCTTGCGCCTTTATTTCCGAAGC
TTCGGTCATTACTTTCTACGGAGGTTGTGGCTTTTAAGCCAAAGGTTTCGGAAGGATATG
AAGTGTTAGGATTTTCGGTCAATACAGATTTAGGTCGAGGTTCTTTTTGCATGATAGAGG
AGATTACCTATCATTATGACCCCAGTGTAAAGTTTAGATGGAGAAGAAGACAGGGAGGAG
TTTGGAGATCATTAGTGGGATTAGAAGGGCTACCTAAGTTTGCTAGATATAGTAGTGTTA
AGTTGGCGGATTGTGGTGGTAAGCTGGTTGTTTTGTGGCACAAGTATGTGCCTGCAAGTG
GGTATGAGGAGAAGATGGTTTGGTGCGCAGAAATTTCCCTTGAAAAGCGCAGCAAGAAAG
AGATTTGGGGGAAGATCGAGTGGTTCGATGCAGTTCTTAGAGTCCCTAAGTATTACCAAT
TCGTTGGGGCTAA

Last Updated on 06.12.2018 16:11