Sequences from confirmation amplicon


Confirmation sequences for line 509D03 and hit At2g22870 are shown.
Line ID 509D03
Locus At2g22870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9739761
Primers used for PCR CC77 - 8474, show primer details
   
Sequence Name 40-K105566-0022-509-D03C-CC77
GenBank Accession LN503708 [GenBank]
Sequencing primer gene specific primer, CC77: 5'-CAGAACTAAGCCAATCGAACTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K105566-0022-509-D03C-CC77
TAATTTACAAAATGAACTGAAATATACCAAATTTAGCCAAAGAAAACTGATTGTTTTATC
CAAAAGCAAGAGATCAAAACACATACCCATAACCAGGCAAATCCACAATGTACCAACTCT
TATTCACCAAGAAGTGATTTATAAGCTGAGTCTTCCCTGTTTCACCAAATAAAAATCCAA
ACTTTCAGATTTCCAAAACTTAAAAGGTTCGATTTTTATAACCAAATTGAAAGGAATAAA
AACACAAACCAGGTTTCTTAGAAGTAAGAGCAACTTCTTTCTTACGAACCAAACAATTGA
TAAGCGAAGACTTGCCAACATTAGAACGACCCAAAATCGCAATCTCAGGACGATCATCTT
TAGGACAATCTCTAGCACGAGCACTACTTTTAACAAATTCAACTTCTTTGATTTGTGAAT
GAAAGAACCAATATATTCAATTGAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCC
   
Sequence Name 72-K105567w-0022-509-D03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K105567w-0022-509-D03C-8409
TTTTCATTGAATATATTGGTTCTTTCATTCACAAATCAAAGAAGTTGAATTTGTTAAAAG
TAGTGCTCGTGCTAGAGATTGTCCTAAAGATGATCGTCCTGAGATTGCGATTTTGGGTCG
TTCTAATGTTGGCAAGTCTTCGCTTATCAATTGTTTGGTTCGTAAGAAAGAAGTTGCTCT
TACTTCTAAGAAACCTGGTTTGTGTTTTTATTCCTTTCAATTTGGTTATAAAAATCGAAC
CTTTTAAGTTTTGGAAATCTGAAAGTTTGGATTTTTATTTGGTGAAACAGGGAAGACTCA
GCTTATAAATCACTTCTTGGTGAATAAGAGTTGGTACATTGTGGATTTGCCTGGTTATGG
GTATGTGTTTTGATCTCTTGCTTTTGGATAAAACAATCAGTTTTCTTTGGCTAAATTTGG
TATATTTCAGTTCATTTTGGTAAAATTTAGGATAAGAGTTCGGTATTTTTGGTTTGGTAG
TTCGATTG
Primers used for PCR 61xd - 3144, show primer details
   
Sequence Name 28-K118953-0022-509-D03C-61xd
GenBank Accession LN499065 [GenBank]
Sequencing primer gene specific primer, 61xd: 5'-TATTTTTGAAAAGCTCCCACTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118953-0022-509-D03C-61xd
AGAGTAGTCAACAATCATTTGTAATTGTAATAGAAAAAACTAGTTTAATTAGAAATTGAG
CATCAAATTTAAACAGCTCTGTAAAATTTTCTAATTGTAATAAGAAATTTTTGTTATATC
TGTCTTAAAATATACACCAAAGGTAGACACAATATCTCCTACAAGTGCACATTCAACTTC
TAGATATAGACAAAGTTCCAAACTTATAATAAGTTTTTTTGCAAAGTTATTGGTTATAAT
TAAGTTTAATTGGTTTATCAATGGGTAAAGTATACAGTAAACTGATTAAACCTAGCTATG
TGGGTTATAGATCTCGAACTATTTACTCGAAGTACATTAAGTTATAGAACTTTCATTTCG
GAGAAAATAAGGATAAAACTTTTATTTTATAGACATATGAACTAGAGTTAAAATATATGA
GCTCTATCTACTGATACTTTTTATGAGAAAATAGACCTTTTTTCTAAAATTATCTTTGAT
GAAAATTTTGTTTGGTACTCCAACATGTGTACAAAAGTTGCGCTAGATCAAAACATATGT
AAGTAACACAACTTATGACATTCTTGATTGCAAAATTAGTTAAAATCTAGTTGTTTTTAT
ATTATTGTTGACACAAAAAAAAATGTCAGCATATGAAAGCCTCCGTCTGAAGCAGAAGGA
ACGACTCTGGTCTAAAACTCTAAAAACCCTATCTTCTTCGTTCTAAACCCAAAACACACA
CAAAACAAAATGGTGCTACTTCTCCGATATCGATCCTTAACGATAAATCTCACTCCTCTA
ATACCCAAATCTCAAAAATTCCACACTCTCCAATCATTTCGTAACCCTAATTTCATCTCC
ATCCCAAAAA
   
Sequence Name 12-K118952-0022-509-D03C-35St
GenBank Accession LN491812 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118952-0022-509-D03C-35St
TCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTCTTTTGATCCGCA
AAAGGTCCGATTACGATATTTGAACCCGGAAGAACCATATCATCTGTTAGTTCCTCAATC
TCTACACCAGGAGGTATGAATAACACTGACTTAGCGAATTTAGTCGCGTCGGAGATCGAC
CGATTCGTTGTTGTTGTTGGATTGTTCGTGGAGGCTGAGATTTTTGGGATGGAGATGAAA
TTAGGGTTACGAAATGATTGGAGAGTGTGGAATTTTTGAGATTTGGGTATTAGAGGAGTG
AGATTTATCGTTAAGGATCGATATCGGAGAAGTAGCACCATTTTGTTTTGTGTGTGTTTT
GGGTTTAGAACGAAGAAGATAGGGTTTTTAGAGTTTTAGACCAGAGTCGTTCCTTCTGCT
TCAGACGGAGGCTTTCATATGCTGACATTTTTTTTTGTGTCAACAATAATATAAAAACAA
CTAGATTTTAACTAATTTTGCAATCAAGAATGTCATAAGTTGTGTTACTTACATATGTTT
TGATCTAGCGCAACTTTTGTACACATGTTGGAGTACCAAACAAAATTTTCATCAAAGATA
ATTTTAGAAAAAAGGTCTATTTTCTCATAAAAAGTATCAGTAGATAGAGCTCATATATTT
TAACTCTAGTTCATATGTCTATAAAATAAAAGTTTTATCCTTATTTTCTCCGAAATGAAA
GTTCTATAACTTAATGTACTTCGA

Last Updated on 06.12.2018 16:11