Sequences from confirmation amplicon


Confirmation sequences for line 126H04 and hit At2g23760 are shown.
Line ID 126H04
Locus At2g23760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10110125
Primers used for PCR 13sm - 8474, show primer details
   
Sequence Name 45-K116549-0022-126-H04C-13sm
GenBank Accession LN505655 [GenBank]
Sequencing primer gene specific primer, 13sm: 5'-TGGCTATTCGAGCATTTTCTTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K116549-0022-126-H04C-13sm
CTACTCTTATTCTTCGATATACTAATCTTCTTGAATGTTGACTATACATTATAAAATGTA
GACTACCATAATGCATTGATACCAAGACTTTAACTAATATACGTTTATTAAACTTAAGTT
ATGAAAGTGACAAAAAAATTTTTTTTTTTATAACTCTTTTGTTATGCGGGAAGATATATT
TCTATTTCAATCAAAATTAAAAGCTGCTGGCTTTTTTAGAAAACCAAAATAATTTTTGTA
AAATGAAGTTCCGTTTCTTCACTGAGTTTGATTATTTTATGTTTATTACTACTAATAAAA
ATTTAAATATCAAATATTTTTTCCTTTTCATACCACCCATAAAAATGGTCAGCTAAAAGT
ACTCCAAAATAACAAGGGTAACAATTCATTGACTAGGAGACTTTCATGAAAACTGTATGT
TTATATTTATATTAATGTAGGTTATATTGCATGGCAAATTGAATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 05-K116548-0022-126-H04C-8409
GenBank Accession LN488510 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K116548-0022-126-H04C-8409
ATTTACATTGAATATTCAATTTGCCATGCAATATAACCTACATTAATATCAATATAAACA
TACAGTTTTCATGAAAGTCTCCTAGTCAATGAATTGTTACCCTTGTTATTTTGGAGTACT
CTTAGCTGACCATTCTTATGGGTGGTATGAAAAGGAAAAAATATTTGATATTTAAATTTT
TATTAGTAGTAATAAACATAAAATAATCAAACTCAGTGAAGAAACGGAACTTCATTTTAC
AAAAATTATTTTGGTTTTCTAAAAAAGCCAGCAGCTTTTAATTTTGATTGAAATAGAAAT
ATATCTTCCCGCATAACAAAAGAGTTATAAAAAAAAAAATTTTTTTGTCACTTTCATAAC
TTAAGTTTAATAAACGTATATTAGTTAAAGTCTTGGTATCAATGCATTATGGTAGTCTAC
ATTTTATAATGTATAGTCAACATTCAAGAAGATTAGTATTATCGAAGAATAAGAGTAGGT
AAGTATTATTAAGGGATTAGAAAATGGACTTACGGATTAAGAAAATGCTCGAAATAGCCA

Last Updated on 06.12.2018 16:11