Sequences from confirmation amplicon


Confirmation sequences for line 819G09 and hit At2g26530 are shown.
Line ID 819G09
Locus At2g26530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11283818
Primers used for PCR AH32 - 8474, show primer details
   
Sequence Name 47-K105566-0022-819-G09C-AH32
GenBank Accession LN506200 [GenBank]
Sequencing primer gene specific primer, AH32: 5'-CTTGTTCTTGTTCTTGTTCTTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K105566-0022-819-G09C-AH32
CGAAGTGACGAGCAACTCTACGACCAGAGTTTTGACGTCTCCCTCTCCCTCTTTCCTCTC
CTAAGCCATTCCTAGCTTTATCCATGGCGACCTCAAAAGGATCCACGTTATCCGGTTTCT
TTCTTGGGGAAAAAGCTTTGCGGATAATGTTCTTACCATGAGCAATAGGTGATCTTGGAG
ACCTTGGAGATAAAATCTGAGGCTGATGATGATGGTCTAATTGTAAATACGGAGGAGGCT
TCAAGGGTTTGATTTTGCCTCCATCAAAAAGCTCTTCAGCAAAAAGCGACGTCGTTTCGA
GTTTCCCACCAATCTCGAAAGCGAAATCAATATCATCATCATCATCGTTGGTGATCTTCC
TAGGCGTGCCTGGTGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCC
   
Sequence Name 79-K105567w-0022-819-G09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K105567w-0022-819-G09C-8409
GCCATTTACATTGAATCCCAGGCACGCCTAGGAAGATCACCAACGATGATGATGATGATA
TTGATTTCGCTTTCGAGATTGGTGGGAAACTCGAAACGACGTCGCTTTTTGCTGAAGAGC
TTTTTGATGGAGGCAAAATCAAACCCTTGAAGCCTCCTCCGTATTTACAATTAGACCATC
ATCATCAGCCTCAGATTTTATCTCCAAGGTCTCCAAGATCACCTATTGCTCATGGTAAGA
ACATTATCCGCAAAGCTTTTTCCCCAAGAAAGAAACCGGATAACGTGGATCCTTTTGAGG
TCGCCATGGATAAAGCTAGGAATGGCTTAGGAGAGGAAAGAGGGAGAGGGAGACGTCAAA
ACTCTGGTCGTAGAGTTGCTCGATCACTTTCGCCTTTTCGGGTTTCGGCTTATCCATGGG
AAGAACAA

Last Updated on 06.12.2018 16:11