Sequences from confirmation amplicon


Confirmation sequences for line 705C05 and hit At2g26570 are shown.
Line ID 705C05
Locus At2g26570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11298643
Primers used for PCR 58zl - 8474, show primer details
   
Sequence Name 91-K130404-0022-705-C05C-58zl
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 58zl: 5'-CTTTATATCGTTCTCACATCCGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K130404-0022-705-C05C-58zl
ATTTTCACCGCTAAACAATCNGTTAAACTGCTTGATAACATTTAAAACCGCAACCGTCCA
CTTTTGTATACTCCTGTAATTTTATCCACATTGGCTGCGTTTAAACCAATCACACTAAGT
TCCAAACAAATTCTTTCTTATACTGAGTATGACCATCGTTTTTAGTTGTAAAGAAACAAA
TTTTTTGTTTTTGATTTAATCTTACTATTCTGTTGTATTATGTTTTGGGCTTTTGAGTTT
ATTTGTATTATGTTTTGTATATATGTTTAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 87-K130403-0022-705-C05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K130403-0022-705-C05C-8409
CATTTACAATTGAATATATCCTAAACATATATACAAAACATAATACAAATAAACTCAAAA
GCCCAAAACATAATACAACAGAATAGTAAGATTAAATCAAAAACAAAAAATTTGTTTCTT
TACAACTAAAAACGATGGTCATACTCAGTATAAGAAAGAATTTGTTTGGAACTTAGTGTG
ATTGGTTTAAACGCAGCCAATGTGGATAAAATTACAGGAGTATACAAAAGTGGACGGTTG
CGGTTTTAAATGTTATCAAGCAGTTTAAACGATTGGTTTAGCGGTTGAAAATATATGCTT
TGTAAAAATTATATAATTGATAAAATAGCGGATGTGAGAACNATATAAAG

Last Updated on 06.12.2018 16:11