Sequences from confirmation amplicon


Confirmation sequences for line 785G05 and hit At2g27150 are shown.
Line ID 785G05
Locus At2g27150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11604104
Primers used for PCR 15ay - 8474, show primer details
   
Sequence Name 46-K112758-0022-785-G05C-15ay
GenBank Accession LN505913 [GenBank]
Sequencing primer gene specific primer, 15ay: 5'-ACCAAGTTTCAAACACACAAAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K112758-0022-785-G05C-15ay
TCTGGAACAGTTTTGAGATACCTCAGAGGAGATTATCTTTCGCTCAGCTTCTTCCATTCC
TTTTATAACATCACCTACTGGTTCGGGGTAAAACATCGGATGAACCTCGAAAAAGCTAGA
TCTTTTAACTGCATCTTCGACAGTTAATATCGGTTGTTCTAAATTCTTTGTATCATACTC
AACTACTGCAAGTTTTGCAGCCATGTCTGCATGTTTCTGTGTGTCTGCAACCTGCAAAAG
CCATATACTTCTCTAGATACGCAACGAAATACGAGCAAGAATTAAAAAAGAAGAGGAAAC
TGGAGTTACCACAAGAGCAATTCTTTGACCTGCACATCGAGTTAGTTCATCTGCAAATAA
AGGTCCTGGTCCAAAAAGGGTCTTGGAACCAATGTTTTGTCCTTGTTGTGGAATATCCTT
GAAAGTAAGAACAGCAAAAACTCCAGTAGGAGTTACATTCTCTCTGAAGCTTAAGCTTTT
TATCTTAGCCAAAGGCTCTGTGCTATAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGC
   
Sequence Name 06-K112757-0022-785-G05C-8409
GenBank Accession LN488887 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K112757-0022-785-G05C-8409
GCATTTATGCACAGAGCCTTTGGCTAAGATAAAAGCTTAAGCTTCAGAGAGAATGTAACT
CCTACTGGAGTTTTTGCTGTTCTTACTTTCAAGGATATTCCACAACAAGGACAAAACATT
GGTTCCAAGACCCTTTTTGGACCAGGACCTTTATTTGCAGATGAACTAACTCGATGTGCA
GGTCAAAGAATTGCTCTTGTGGTAACTCCAGTTTCCTCTTCTTTTTTAATTCTTGCTCGT
ATTTCGTTGCGTATCTAGAGAAGTATATGGCTTTTGCAGGTTGCAGACACACAGAAACAT
GCAGACATGGCTGCAAAACTTGCAGTAGTTGAGTATGATACAAAGAATTTAGAACAACCG
ATATTAACTGTCGAAGATGCAGTTAAAAGATCTAGCTTTTTCGAGGTTCATCCGATGTTT
TACCCCGAACCAGTAGGTGATGTTATAAAAGGAATGGAAGAAGCTGAGCGAAAGATAATC
TCCTCTGAGGTATCTCAAAACTGTTTCCAGATTTTCACCGCCNAAGTTGTGTAGTGACAT
CTCATTTTGTGTGTTTGAAACTTGG

Last Updated on 06.12.2018 16:11