Sequences from confirmation amplicon


Confirmation sequences for line 662B07 and hit At2g27190 are shown.
Line ID 662B07
Locus At2g27190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11623484
Primers used for PCR NG92 - 8474, show primer details
   
Sequence Name 91-K102372-0022-662-B07C-8409
GenBank Accession LN515110 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K102372-0022-662-B07C-8409
ATTTACAATTGAATAAAGCACGTTCTTCGTCTCGGATTCAGAGAAACTAATCTTGAAGAT
GAGTTCAAGATCTGACCTCAAAATCAAACGAGTTTCTTTGATAATCTTCTTGCTAAGTGT
TCTTGTTGAGTTTTGCTATGGAGGGTTCACAAGTGAATATGTCAGAGGAAGTGATTTACC
AGATGACATGCCACTAGACAGCGATGTTTTTGAAGTTCCTCCTGGTCCTAATTCACCTCA
ACAGGTTCATGTAACGCAAGGGAACCATGAAGGTAATGGAGTGATCATTTCTTGGGTGAC
TCCGGTTAAGCCCGGTTCTAAGACTGTTCAATATTGGTGTGAGAATGAGAAATCGAGGAA
ACAAGCAGAAGCAACAGTTAATACTTACCGGTTCTTTAATTACACGTCTGGTTATATTCA
CCATT
   
Sequence Name 91-K102373-0022-662-B07C-NG92
GenBank Accession LN515111 [GenBank]
Sequencing primer gene specific primer, NG92: 5'-CAATGGTGAATATAACCAGACGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K102373-0022-662-B07C-NG92
GTTGCTTCTGCTTGTTCCTCGATTTCTCATTCTCACACCAATATTGAACAGTCTTAGAAC
CGGGCTTAACCGGAGTCACCCAAGAAATGATCACTCCATTACCTTCATGGTTCCCTTGCG
TTACATGAACCTGTTGAGGTGAATTAGGACCAGGAGGAACTTCAAAAACATCGCTGTCTA
GTGGCATGTCATCTGGTAAATCACTTCCTCTGACATATTCACTTGTGAACCCTCCATAGC
AAAACTCAACAAGAACACTTAGCAAGAAGATTATCAAAGAAACTCGTTTGATTTTGAGGT
CAGATCTTGAACTCATCTTCAAGATTAGTTTCTCTGAATCCGAGACGAAGAACGTGCTTT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAGG
NTCAATATAGT

Last Updated on 06.12.2018 16:11