Sequences from confirmation amplicon


Confirmation sequences for line 263G04 and hit At2g28671 are shown.
Line ID 263G04
Locus At2g28671 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12301430
Primers used for PCR NC28 - 8474, show primer details
   
Sequence Name 31-K101218-0022-263-G04C-8409
GenBank Accession LN500087 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K101218-0022-263-G04C-8409
AACTGGCAGTTGGTAGAAGACCAGGACCTGAACCACTTGATGGTATTGACCCTGTACCTG
CACCGAGACCGCTTCCAGTAGAACCTGAACCAGATGACCCTGATCCTGCACCAAAACCGA
ATCCCGTGGAACCTGAACCACTTGACCCTGTTCCTGCACCAAAACCAATTCCGGTGGAAC
CTGAACCTGAGCCAAGGGTGGTTGTGGTAGCTGAGAATGCATTTTCTTCATCAAGAAGCC
GAGCCGCAGCTGCAAAAGTAACAACAAGAGCTATAACCAGTAAAGAAAGTATCTTTTGAC
CAGCCATTGAACAAGAAAGATGATCACTCAGAGAGAAGCAAAGATTGTTGTTTGAAGTAA
ATGATTGTGATGATAGAATCAAATTCTCAAAGAGTATATATACATACATGAAATGAGATA
GTTAATACAAGAAGATGGAGTTGATGAAATG
   
Sequence Name 31-K101225-0022-263-G04C-NC28
GenBank Accession LN500088 [GenBank]
Sequencing primer gene specific primer, NC28: 5'-CATTTCATCAACTCCATCTTCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K101225-0022-263-G04C-NC28
CTCTTTGAGATTTGATTCTATCATCACAATCATTTACTTCAACAACAATCTTTGCTTCTC
TCTGAGTGATCATCTTTCTTGTTCAATGGCTGGTCAAAAGATACTTTCTTTACTGGTTAT
AGCTCTTGTTGTTACTTTTGCAGCTGCGGCTCGGCTTCTTGATGAAGAAAATGCATTCTC
AGCTACCACAACCACCCTTGGCTCAGGTTCAGGTTCCACCGGAATTGGTTTTGGTGCAGG
AACAGGGTCAAGTGGTTCAGGTTCCACGGGATTCGGTTTTGGTGCAGGATCAGGGTCATC
TGGTTCAGGTTCTACTGGAAGCGGTCTCGGTGCAGGTACAGGGTCAATACCATCAAGTGG
TTCAGGTCCTGGTCTTCTACCAACTGCCAGTTCTGTTCCTGGTTCTACATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCACA

Last Updated on 06.12.2018 16:11