Sequences from confirmation amplicon


Confirmation sequences for line 737A02 and hit At2g29560 are shown.
Line ID 737A02
Locus At2g29560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12649100
Primers used for PCR KA13 - 8474, show primer details
   
Sequence Name 64-K105140-0022-737-A02C-KA13
GenBank Accession LN511200 [GenBank]
Sequencing primer gene specific primer, KA13: 5'-GCATTAGCCAAAAGTTGAAGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K105140-0022-737-A02C-KA13
TGCTAGATTCCATACCACACATAACTCAGCTACTTAAAAGCTAAAAAGAAGCTGATGATA
ATCTGATGAAAGAGCTATGATCCAAAATTCCAATGCAGGTTACTCCACGTAATGTAGATT
GTTCTAGTGTAATTGCATAAGGGGTACTGAGGATATTAGTTTGGAAAAAGATCCAGACCT
GGTTATACTTCATAGTACGTTCTCCTCTGCAAGGAGCACCAGCTTTAATCACACCTGTTG
CGAGACCCACAGATAAGTCAGAGATGAAAGAGTCCTCTGTTTCTCCACATCTATGAGATG
TCACCACACCCCACTGGGCATCCCTTGCCATTTTCACTACTTCAATCTGATTCAATTGGT
TTATAAATATACCCGCCAATCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGC
   
Sequence Name 48-K105141-0022-737-A02C-8409
GenBank Accession LN506503 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K105141-0022-737-A02C-8409
GCGGGTATATTTATAAACCAATTGAATCAGATTGAAGTAGTGAAAATGGCAAGGGATGCC
CAGTGGGGTGTGGTGACATCTCATAGATGTGGAGAAACAGAGGACTCTTTCATCTCTGAC
TTATCTGTGGGTCTCGCAACAGGTGTGATTAAAGCTGGTGCTCCTTGCAGAGGAGAACGT
ACTATGAAGTATAACCAGGTCTGGATCTTTTTCCAAACTAATATCCTCAGTACCCCTTAT
GCAATTACACTAGAACAATCTACATTACGTGGAGTAACCTGCATTGGAATTTTGGATCAT
AGCTCTTTCATCAGATTATCATCAGCTTCTTTTTAGCTTTTAAGTAGCTGAGTTATGTTG
TGGTATGTGAATCTTTAGCAACCTAGGCTAAGTTTCAGTTTCTTCAACTTTGGCCTAAT

Last Updated on 06.12.2018 16:11