Sequences from confirmation amplicon


Confirmation sequences for line 076F04 and hit At2g31865 are shown.
Line ID 076F04
Locus At2g31865 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13547115
Primers used for PCR BD28 - 8474, show primer details
   
Sequence Name 31-K028097-022-076-F04C-8409
GenBank Accession LN500022 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K028097-022-076-F04C-8409
GTTTTTCGCAGTTAAATAGAAAGATAATTATTGTTTCGTAAGAATAAGTTGATTGAAAAC
AGAGAAGCATGCCTGTGAAATGGCAAGCCACTGAAGCATGATCTTTAGTTCTGGATCACC
TCCAAACACACCGCAGCCCCAGTTCCCTGTAGCTACACCAATCTTTTTCTCTTCATGATC
GATACACCATCTATGAGAGCTTTCTATAACTTGGCTCGCAGAATCTGATGTAAGAGGAAC
ATGCTGCTTTGCGAAAACAAATTTAATCTTGGTCTAGTCAAGAAAAACAAAATGCTTAAC
ATGTATGCTAAATTCATGTATGGAG
   
Sequence Name 31-K028097-022-076-F04C-BD28
GenBank Accession LN500023 [GenBank]
Sequencing primer gene specific primer, BD28: 5'-ACCACAATTATGCTCCATACATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K028097-022-076-F04C-BD28
GCATTTTGTTTTTCTTGACTAGACCAAGATTAAATTTNGTTTTCGCACAGCAGCATGTTC
CTCTTACATCAGATTCTGCGAGCCAAGTTATAGAAAGCTCTCATAGATGGTGTATCGATC
ATGAAGAGAAAAAGATTGGTGTAGCTACCGGGAACTGGGGCTGCGGTGTGTTTGGAGGTG
ATCCAGAACTAAAGATCATGCTTCAGTGGCTTGCCATTTCACAGGCATGCTTCTCTGTTT
TCATCAACTTATCTTACAAAACATAATTATCATTCTATTTAACTGCGAAAACAGCAATCT
TTGAGTATGATGGATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11