Sequences from confirmation amplicon


Confirmation sequences for line 127H02 and hit At2g31960 are shown.
Line ID 127H02
Locus At2g31960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13600196
Primers used for PCR RK47 - 8474, show primer details
   
Sequence Name 21-K016206-022-127-H02C-8409
GenBank Accession LN495448 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K016206-022-127-H02C-8409
ATTTCCCGGACATGGTTCTTACTATTTGATTTTGTTTCGGTTCGGCTTACTTGGTTCTTA
AATCTTCGGTTTAAGGAAATAGCGATAGTGTAGATCGGTTTGGCAAGGTTTTGACTTTTG
ATTGCATTGGTTCTATTTTGTATTAAATAAAAGATAAGTCCCTGAAATTGTAGTTGTTTT
CAAAATACTCAAAATATTTTAGTACGCTTCTAATCTAACCCCAAAAGGAAGAAACTGTAT
ACGACCGGAGGTTCTCAATCGAATACTACACCGGTTCAATTCGATTCTGTTTATAAACCG
GTCTCTCGCGATTCAATTCGTCTTCTTTTTCCAATTTCCAGTTCCTTCTTCTTCCATTGT
CGAGTTTTCATGATTTTCCCGCAAAACCCTTCTCGTTTATGGGTTTAACCTTCCTGTGTC
ACCAAAATTTTCTCCGTAACTTATCACCTCCACTTTTTCATATCTGTAATCGCTGAAGGT
AAAAGATGAGTACGGTCGATAAAATGTTGATCAAGGGTATAAGAAGCTTCGACCCAGAAA
ATAAGAACGTTGTTACTTTCTTCAGACCTTTAACCCTAATCGTCGGCGCTAACGGAGCCG
GAAAAACTGTAAAGTTCCTTAAACCTTTTCTCTTCTCCTCAGTTCACTCCCATTTCTTCG
CCTCCGTAATTGAAAAGTTTGTGGCTATTTCGTGATTTTTTG
   
Sequence Name 22-K016206-022-127-H02C-RK47
GenBank Accession LN495890 [GenBank]
Sequencing primer gene specific primer, RK47: 5'-TTGTACTCCATCTTTGAAGCCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K016206-022-127-H02C-rk47
TCAACTGAAATGACCTTATACATACCACATCCTTCCCTGCAGCTGTCTTGAATCTCAGCT
TTATTTGTGCTTTTGTCTCCGTTTCTCCAGCAACCTATATAGTAGGAACCAATGAAATTT
CAATCTGAATACTACTATAAGGCGAAATCGAATCCTGAATTCCACATTCATCAACAACAG
AAACATGAAATTAGGTAAGAATTGAGAATCTAACTAGAGACTTACCTTAGGGTCATGAAT
GAAGCTATGACCAGACCTCGCGTTCGGTGGCAACTCTCCGGTACAAGACACCTTTAAACA
CTCTATAATCGTCTGCAAAAATCACGAAATAGCCACAAACTTTTCAATTACGGAGGCGAA
GAAATGGGAGTGAACTGAGGAGAAGAGAAAAGGTTTAAGGAACTTTACAGTTTTTCCGGC
TCCGTTAGCGCCGACGATTAGGGTTAAAGGTCTGAAGAAAGTAACAACGTTCTTATTTTC
TGGGTCGAAGCTTCTTATACCCTTGATCAACATTTTATCGACCGTACTCATCTTTTACCT
TCAGCGATTACAGATATGAAAAAGTGGAGGTGATAAGTTACGGAGAAAATTTTGGTGACA
CAGGAAGGTTAAACCCATAAACGAGAAGGGTTTTGCGGGAAAATCATGAAAACTCGACAA
TGGAAGAAGAAGGAACTGGAAATTGGAAAAAGAAGACGAATTGAATCGCGAGAGACCGGT
TTATAAACAGAATCGAAT

Last Updated on 06.12.2018 16:11