Sequences from confirmation amplicon


Confirmation sequences for line 365G07 and hit At2g33610 are shown.
Line ID 365G07
Locus At2g33610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14228948
Primers used for PCR JL14 - 8474, show primer details
   
Sequence Name 42-K032417-022-365-G07C-8409
GenBank Accession LN504354 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K032417-022-365-G07C-8409
TTACATTGAATATATCCTGATCTACTTCTCCGGCGAAGTTGCGTTAGTTAAACAGAGAGT
CATGGCCATGAAAGCTCCCGATCCCGGCGGATCTGGGGAAATTCTCCCGAGCACTCCCTC
TCTTTCCGAAACTACGTCTGGAGGCGCCGCAGCAGCTTCTAAGTCGGCTCAATTGCCGTC
TTCTTCTTCCGATATCGATAATATTCACGTACCTAGCTACTCCAGTAAGTCTGCAACGCC
GAGAAAACTTTGATTTATTCTACTTTCTTCACTGTTTCTTACGGATTTTGTACTTGTACC
GTCTATTTAACCAATTAGGTTGGTTTTCATGGACCGACATTAACGATTGCGAGGTCCGGT
CACTGCCGGAATTTTTCGATTCGAGATCTTCATCCAAAAACCCTAAGTTTTATCTCTACT
TGAGGAACTCGATTATTAAGCAGTACAGAGACGACCATCCTCGGAAGATTATTTTCCACT
GA
   
Sequence Name 42-K032417-022-365-G07C-JL14
GenBank Accession LN504355 [GenBank]
Sequencing primer gene specific primer, JL14: 5'-GTCAGTGAAACTAATCTTCCGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K032417-022-365-G07C-JL14
TCGAGTTCTCAAGTAGAGATAAAACTTAGGGTTTTTGGATGAAGATCTCGAATCGAAAAA
TTCCGGCAGTGACCGGACCTCGCAATCGTTAATGTCGGTCCATGAAAACCAACCTAATTG
GTTAAATAGACGGTACAAGTACAAAATCCGTAAGAAACAGTGAAGAAAGTAGAATAAATC
AAAGTTTTCTCGGCGTTGCAGACTTACTGGAGTAGCTAGGTACGTGAATATTATCGATAT
CGGAAGAAGAAGACGGCAATTGAGCCGACTTAGAAGCTGCTGCGGCGCCTCCAGACGTAG
TTTCGGAAAGAGAGGGAGTGCTCGGGAGAATTTCCCCAGATCCGCCGGGATCGGGAGCTT
TCATGGCCATGACTCTCTGTTTAACTAACGCAACTTCGCCGGAGAAGTAGATCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTAC
Primers used for PCR JL13 - 8474, show primer details
   
Sequence Name 48-K032418-022-365-G07C-8409
GenBank Accession LN506415 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K032418-022-365-G07C-8409
GGCAAGTTAAAAAAAAGTCGGATATCTAAACGGGCCTGGTTTGGGCCCTAATACAGGTCC
ATTAACCTGGCTTTAGTTGTTAAATATTGCGAATGTTTTTTTAGTCCCATGGTCAGCCAA
GATGTAGTGCATCTGCTACGCTAGGCTTAGAGGTTAATTCGAATGGTGAAGTTGTGCGTT
ACTGATTTCATGTATTAATGGTGAAGTTATCCTTTGTAAGTAGGTAGAAATTGGAACGTA
TCAAAGAGATATTGGAACATAGTTATGGAAGGAGTAATTGTAGATTGGTCATAAAAGTAA
TGGTGTCTAAGAAAGTAACAAAAACTCAGAAGTTCATGATGGAAAATATGTTGCACAACT
CTTATTCTTGAGTAAAAGATGAAAGAGCGTGTGCAGATAGGAACACTTAACACATGGACC
CAAAACAC
   
Sequence Name 48-K032418-022-365-G07C-JL13
GenBank Accession LN506416 [GenBank]
Sequencing primer gene specific primer, JL13: 5'-ATAGCATATGAGTGTTTTGGGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K032418-022-365-G07C-JL13
GCTCTTTCATCTTTTATCAGAATAAGAGTTGTGCAACATATTTTCCATCATGAACTTCTG
AGTTTTTGTTACTTTCTTAGACACCATTACTTTTATGACCAATCTACAATTACTCCTTCC
ATAACTATGTTCCAATATCTCTTTGATACGTTCCAATTTCTACCTACTTACAAAGGATAA
CTTCACCATTAATACATGAAATCAGTAACGCACAACTTCACCATTCGAATTAACCTCTAA
GCCTAGCGTAGCAGATGCACTACATCTTGGCTGACCATGGGACTAAAAAAACATTCGCAA
TATTTAACAACTAAAGCCAGGTTAATGGACCTGTATTAGGGCCCAAACCAGGCCCGTTTA
GATATCCGACTTTTTTTTTAACTTTGCCCATCTCGATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACTT

Last Updated on 06.12.2018 16:11