Sequences from confirmation amplicon


Confirmation sequences for line 654G12 and hit At2g34110 are shown.
Line ID 654G12
Locus At2g34110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14395008
Primers used for PCR 24yq - 8474, show primer details
   
Sequence Name 43-K122488-0022-654-G12C-24yq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 24yq: 5'-CCATCATATACTCCTCCCGAGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K122488-0022-654-G12C-24yq
AGACTGGTTGCCTCGATACAGCCTCGAGCAAGGCCCGCTCGTTGTTGCTAGGGACGGTTT
GGAATCCACTGATACGTGCAGCTGGTCTTGCATTGGACCGGCATCCCCCATCTGATCCTT
GGTAAGAGTAGTCATTCTCTGATGCGATGCCTCGATTTTGGACTACATAGTTGAAAGCGT
CCGACATTATCCCACCGTCGCAGCCTCGGTCGTACTCTCTGTCGCAGTCTAGAAGCTGTT
GTTCGGACAGAGATACAAGGTTCCCGCCGGCGATCTTAGCCACACCTTCCACTGCTGCTA
CAGCCGAAAATGCCCAGCAACATCCTGTAGATATGGATATTTGGTAAGTTGGATGCCGAT
CATAATAGAAACGAGACAAAATATAATGACCAAATCATCACTGAACCCTACTTACTATAA
CCCTATAACTAACTTGGACATCAAGTTTAATATTTTGTATCCTCCCAATTGTTTTCGCCT
TTGCATTGTATTTGATTCTAGATATTATACATGCTAAATTGTTATTCTTACTTCTATGAA
CCAAAATGAAACATAATTAATTTAACCATATTTGAAAATTAATTCTAAAAATAAAATTGT
AAATTAACTTGATAATCAATGAAAGAATATTACCGCATTGGCCTTGGTACTTGACAGGAG
TGACAGCTCCTTCAGCTCTCCAGTCCTTGCTCTCGACTACCATGTCACTGACGTTCCAAG
TCTGAGACGATATCGTTTTGGCGACCACCTTGGATGGGGAAACTTCAGTTAGGCCCTTTA
GTCCGGTATGTATTGCTAGGAACTCCTCGTTGGTCCAATCCGCGAATTCATTGACACCAA
GCTTGTAGCTTTTGTTCCCTTTTTTGTTGAAAGTCTCAATGAAACTTCAGTTCTTCTTTA
ACACATCGCGTCTCATATTTTTCTCGAGCTCATC
   
Sequence Name 12-K122487-0022-654-G12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K122487-0022-654-G12C-8409
AGTTTTTTCTTCAAATATCACGGCGCAAGTAATGCAAAGTATTAACATCACCCCAAATGG
ATTCAAGTCAAGTTGCATTTTCTTACATACAATTGGATTTCATGGTTCAATCCTAAATCT
ATAAATACTATTCCTTCTATTCTCACTTCATCACCACATTACAAGTCAACACCATTAATC
ATAAGCCCATATTAAAAACATAATTATTTCCTCTATTTTCCAAAACCTAAATGGCATCAA
TCATGGTATTGGTCACTGTTTTAATCATTCTTTTCACGGGTTTCAGAATCTCTCAAGCTA
CGTCTCGTACTGTCATCTTTCGCGAGCAGTCAATGGTTGATAAACATGAGCAATGGATGG
CTCGATTTTCTCGTGAATACCGTGATGAGCTCGAGAAAAATATGAGACGCGATGTGTTTA
AGAAGAACTTGAAGTTCATTGAGAACTTCAACAAAAAAGGGAACAAAAGCTACAAGCTTG
GTGTCAATGAATTCGCGGATTGGACCAACGAGGAGTTCCTAGCAATACATACCGGACTAA
AGGGCCTAACTGAAGTTTCCCCATCCAAGGTGGTCGCCAAAACGATATCGTCTCAGACTT
GGAACGTCAGTGACATGGTAGTCGAGAGCAAGGACTGGAGAGCTGAAGGAGCTGTCACTC
CTGTCAAGTACCAAGGCCAATGCGGTAATATTCTTTCATTGATTATCAAGTTAATTTACA
ATTTTATTTTTAGAATTAATTTTCAAATATGGTTAAATTAATTATGTTTCATTTTGGTTC
ATAGAAGTAAGAATAACAATTTAGCATGTATAATATCTAGAATCAAATACAATGCAAAGG
CGAAAACAATTGGGAGGATACAAAATATTAAACTTGATGTCCAAGTTAGTTATAGGGTTA
TAGTAAGTAGGGTTCAGTGATGATTTGGTCATTATATTTTGT

Last Updated on 06.12.2018 16:11