Sequences from confirmation amplicon


Confirmation sequences for line 447E03 and hit At2g34150 are shown.
Line ID 447E03
Locus At2g34150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14420926
Primers used for PCR MK23 - 8474, show primer details
   
Sequence Name 87-K031694-022-447-E03C-8409
GenBank Accession LN514711 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K031694-022-447-E03C-8409
CATTTACAATCTCTGGAATAGTTTCTGCTATAATTTTCGGTTTCGATGGTTTCAGTCCTA
GGAGATTTCCATTAGTCCATGTTTTAATGGATTCAGTGTCACAAGAACTCTCAACTTCTT
CTGATTCTTGATGTTCTTGGATACCACTAATATCATACAAAGGATTTGAGAAGCCATTGG
AATAACTATGAGTGCCACTACTTATTTCCGAAGCCGCCCATGGATCTTGATGAATCAGTT
CATCAGATTTATATACCGGTGATGATGATAAATGTAGCTCTGATGTAGCAGCACATTTTG
TCTCATCAACACTAAAGTTGTTAGAAATCTTCTCTGTTTCTTGCTCTGTAATATCATTGT
AAAGATTCTTCTGCACCTCTTTAGTGGCTTGGAAAGCTTCATCATTTTCAGATTCTGAAT
CGATGGTGTAAAGTGCATCAACAAAATCATCCCCTTCGCTTTCAGTCTCATCCTCATTGT
CCATTTCCAGTTCCTTCGGTTTGATATCTTGGACCGCTTCAACCGCTGAATGTTCTTTAA
GTCTTGATACCGGTTCAGCATCCAAGACAAAGTTAGTTTCCATTACTTCAGGAGCTTCA
   
Sequence Name 39-K031694-022-447-E03C-MK23
GenBank Accession LN503250 [GenBank]
Sequencing primer gene specific primer, MK23: 5'-ATGAAGCTCCTGAAGTAATGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K031694-022-447-E03C-MK23
ACGGTATCAGACTTAAAGAACATTCAGCGGTTGAAGCGGTCCAAGATATCAAACCGAAGG
AACTGGAAATGGACAATGAGGATGAGACTGAAAGCGAAGGGGATGATTTTGTTGATGCAC
TTTACACCATCGATTCAGAATCTGAAAATGATGAAGCTTTCCAAGCCACTAAAGAGGTGC
AGAAGAATCTTTACAATGATATTACAGAGCAAGAAACAGAGAAGATTTCTAACAACTTTA
GTGTTGATGAGACAAAATGTGCTGCTACATCAGAGCTACATTTATCATCATCACCGGTAT
ATAAATCTGATGAACTGATTCATCAAGATCCATGGGCGGCTTCGGAAATAAGTAGTGGCA
CTCATAGTTATTCCAATGGCTTCTCAAATCCTTTGTATGATATTAGTGGTATCCAAGAAC
ATCAAGAATCAGAAGAAGTTGAGAGTTCTTGTGACACTGAATCCATTAAAACATGGACTA
ATGGAAATCTCCTAGGACTGAAACCATCGAAACCGAAAATTATAGCAGAAACTATTCCAG
AGATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGT
CTACATT

Last Updated on 06.12.2018 16:11