Sequences from confirmation amplicon


Confirmation sequences for line 645F02 and hit At2g34220 are shown.
Line ID 645F02
Locus At2g34220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14451908
Primers used for PCR NL16 - 8474, show primer details
   
Sequence Name 36-K104096-0022-645-F02C-NL16
GenBank Accession LN502203 [GenBank]
Sequencing primer gene specific primer, NL16: 5'-CACAAGAAGATTTTAGAACCTCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K104096-0022-645-F02C-NL16
TTTCCGCTCGCGATCTTTCGCTTCAGGCCAATCACTACACCACCCATTAACATAAACAAA
CTCTTCCCCGCGCTTAGCTCTATCCTTGATAAGATTTTCTGCCTCCGCTGTATCCACAGG
CTTCCAATCCCCACAGCATATCATGTCAGCCAAAATCTCATCCACACACTTAGGACGAGC
ACTAAAGTCAGGTTCAGAGTTGTGCACATGATTATCTAAGATGTGCATCTTGCAATCCGT
AAAACAGTAGTTAACCTGAGAACAAATGTGGCACTTCCAACATCTCCATCGAAGATTATT
GGCGATGGGTATACATTTCTGAAAGTGCTCTTTTACTTCCGTCCCATGCTTATCTTGTAT
ATAGCCTATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCA
   
Sequence Name 52-K104097-0022-645-F02C-8409
GenBank Accession LN507798 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K104097-0022-645-F02C-8409
GAAGCCATTTATAGGCTATATACAAGATAAGCATGGGGACGGAAGTAAAAGAGCACTTTC
AGAAATGTATACCCATCGCCAATAATCTTCGATGGAGATGTTGGAAGTGCCACATTTGTT
CTCAGGTTAACTACTGTTTTACGGATTGCAAGATGCACATCTTAGATAATCATGTGCACA
ACTCTGAACCTGACTTTAGTGCTCGTCCTAAGTGTGTGGATGAGATTTTGGCTGACATGA
TATGCTGTGGGGATTGGAAGCCTGTGGATACAGCGGAGGCAGAAAATCTTATCAAGGATA
GAGCTAAGCGCGGGGAAGAGTTTGTTTATGTTAATGGGTGGTGTAGTGATTGGCCTGAAG
CGAAAGATCGCGAGCGGGAAAACGTACTCAAACAGTTTGCCGAGGTTCTAAATCTTTCTT
GT

Last Updated on 06.12.2018 16:11