Sequences from confirmation amplicon


Confirmation sequences for line 311D06 and hit At2g35330 are shown.
Line ID 311D06
Locus At2g35330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14869711
Primers used for PCR JL29 - 8474, show primer details
   
Sequence Name 36-K032416-022-311-D06C-8409
GenBank Accession LN502111 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K032416-022-311-D06C-8409
TGCTGATGGTTATTTGTTGCAACAAGTTAAACCTAATTTGAGTAAAGGTGATGCAATGTG
GTGTTTGCTAATGAGTGAGCTTCATGTTGGGAGGGCGAGTACTATGGATATACCGAGTTC
TGGAAAAGGGGATAGTAGTAATGTAGGGGTTGGTGGTGCTAGTAGTACTGTAAATGGGGT
TGGTGGTGCTATTGCTCCGGCATTGTGTAGATTCCATGGAGGTTGGGGTTTTGGTAATGG
GAAAGGACCCAAGTTTTCTGGTAATGGGTTTTCTTTGCACAGTGAAGAGTTAACGTTGCA
GAGAGAGATTGATTGTCCAAGGAGATTTAATCTCTCACCTTCGATGAAGTCTTTGTTGAG
AGAGAATGTTGCAGCCTTTGCTGCTGGGTATCGTGCTAGTATGGAGCAAAAGAAGCAGGT
GCAAATGCAGTCTGAAACTAGTGGTACTAGCTTGTCTTGCACGGCTGCTGCTACACATTC
AGAGAAGTGTGAGCAGCCACATGTCTTCGGAAGTGAGAAAATGTT
   
Sequence Name 36-K032416-022-311-D06C-JL29
GenBank Accession LN502112 [GenBank]
Sequencing primer gene specific primer, JL29: 5'-GAAACATTCTTCACTTCCGAAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K032416-022-311-D06C-JL29
CAGCAGCCGTGCAAGACAAGCTAGTACCACTAGTTTCAGACTGCATTTGCACCTGCTTCT
TTTGCTCCATACTAGCACGATACCCAGCAGCAAAGGCTGCAACATTCTCTCTCAACAAAG
ACTTCATCGAAGGTGAGAGATTAAATCTCCTTGGACAATCAATCTCTCTCTGCAACGTTA
ACTCTTCACTGTGCAAAGAAAACCCATTACCAGAAAACTTGGGTCCTTTCCCATTACCAA
AACCCCAACCTCCATGGAATCTACACAATGCCGGAGCAATAGCACCACCAACCCCATTTA
CAGTACTACTAGCACCACCAACCCCTACATTACTACTATCCCCTTTTCCAGAACTCGGTA
TATCCATAGTACTCGCCCTCCCAACATGAAGCTCACTCATTAGCAAACACCACATTGCAT
CACCTTTACTCAAATTAGGTTTAACTTGTTGCAACAAATAAACCATCAGCAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACT
Primers used for PCR JL30 - 8474, show primer details
   
Sequence Name 40-K032417-022-311-D06C-8409
GenBank Accession LN503606 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K032417-022-311-D06C-8409
CGGACTGAGCCTTTACAATTGAAATATCCTGATCCTCATTGTTCACATTACTACCTTCAC
CAGTATTACTCTTAAGATAAGCCAACGAATTATGAAGAATATTAGTCATAACATCCATTC
CACCATAACAATACCCATTACTAAGAACAGCTCTCAACGCCACATCCTCATCATAACCAG
AACCCACAAGCTTCGAAATCGCTTCATTGTATAAATACTCCAAATGTTTCAACAAAATAT
CCTCTAATTGCTCCTCCGTACAATAACCCCAACCATTATTATCCTCAAAACTGCCACTAG
GGTTTGACTCTGGACACGAATCGAGAAGACCCGGATGATAAACAAGATGCTTAAGACTCG
ATTCAACAATCGATTTCGATAGAGCCGTCCTGTCGAGCAAACAACACGGATCGAATTCGG
GTTTCACGGATCTGGTCTTCCGGTTTGGTCTAACATGCTTCTCCCTCACAGTACAACCCA
TCAGAGTTAAAGCACGTCTATGTATATATGAGAATCTGAAGGAAAAGAAAAATTCCACAG
AGATTGAAAGAGTGAAGATCATTGAATCCCCACAAATGAAACACAAAAACCAAAGCAGGA
TCAGGAGAAATGGGTTTTAATT
   
Sequence Name 40-K032417-022-311-D06C-JL30
GenBank Accession LN503607 [GenBank]
Sequencing primer gene specific primer, JL30: 5'-ATCATTTTGCCATCAATTAAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K032417-022-311-D06C-JL30
GTTTTGTGTTTCATTTGTGGGGATTCATGATCTTCACTCTTTCATCTCTGTGGAATTTTT
CTTTTCCTTCAGATTCTCATATATACATAGACGTGCTTTAACTCTGATGGGTTGTACTGT
GAGGGAGAAGCATGTTAGACCAAACCGGAAGACCAGATCCGTGAAACCCGAATTCGATCC
GTGTTGTTTGCTCGACAGGACGGCTCTATCGAAATCGATTGTTGAATCGAGTCTTAAGCA
TCTTGTTTATCATCCGGGTCTTCTCGATTCGTGTCCAGAGTCAAACCCTAGTGGCAGTTT
TGAGGATAATAATGGTTGGGGTTATTGTACGGAGGAGCAATTAGAGGATATTTTGTTGAA
ACATTTGGAGTATTTATACAATGAAGCGATTTCGAAGCTTGTGGGTTCTGGTTATGATGA
GGATGTGGCGTTGAGAGCTGTTCTTAGTAATGGGTATTGTTATGGTGGAATGGATGTTAT
GACTAATATTCTTCATAATTCGTTGGCTTATCTTAAGAGTAATACTGGTGAAGGTAGTAA
TGTGAACAATGAGGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATNTCGACGTGTCT

Last Updated on 06.12.2018 16:11