Sequences from confirmation amplicon


Confirmation sequences for line 371C12 and hit At2g36800 are shown.
Line ID 371C12
Locus At2g36800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15425284
Primers used for PCR HF02 - 8474, show primer details
   
Sequence Name 01-K024506-022-371-C12C-8409
GenBank Accession LN486416 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K024506-022-371-C12C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAGAAGAAAGAGTGAT
AGCTACATCTATCGTCAAGTCTCAAAAATCAAAATAAACATAGAGGCCACATAAATAAAT
TACCAATGTTACCAAAATCTAATGTGTGTTTGAAACTTCAAAGACCGTTTAAACTCTCAT
ATGAAACACTAACTGAAGATAAATCAAACGTTGGACTCTGTCAAGACAAGTATATCCAAC
ACTAGCCTCAAAAATTATAATCAATAAAAAGAGGACCACAGCCTACAACAATTTCGTGTG
AATGTTTCTTTCCTGAGTTTGGTAATAGAGATGACACACGAGTGACAATATCCTTTACAA
GATGAAAACATAATACACTTTACAACAAG
   
Sequence Name 02-K024506-022-371-C12C-HF02
GenBank Accession LN486872 [GenBank]
Sequencing primer gene specific primer, HF02: 5'-GGTTTTTGTGCTTGTTGTAAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K024506-022-371-C12C-hf2
TGTTTTCATCTTGTAAAGGATATTGTCACTCGTGTGTCATCTCTATTACCAAACTCAGGA
AAGAAACATTCACACGAAATTGTTGTAGGCTGTGGTCCTCTTTTTATTGATTATAATTTT
TGAGGCTAGTGTTGGATATACTTGTCTTGACAGAGTCCAACGTTTGATTTATCTTCAGTT
AGTGTTTCATATGAGAGTTTAAACGGTCTTTGAAGTTTCAAACACACATTAGATTTTGGT
AACATTGGTAATTTATTTATGTGGCCTCTATGTTTATTTTGATTTTTGAGACTTGACGAT
AGATGTAGCTATCACTCTTTCTTCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
Primers used for PCR HF01 - 8474, show primer details
   
Sequence Name 17-K024507-022-371-C12C-8409
GenBank Accession LN493696 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K024507-022-371-C12C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTATTTCAATTGAATTTATCATTGTGAGC
CCTCCAAGTTGTTGTTTTGTGTATGTGAATTATCTTCGTTTTCTTTATGAAGATATGTTT
CTAAACTTTTCCTGAGAAGGAAGGTCACTACGATGGTCACATACGGACAAACTAAGTAGC
AAGCACAATCTGTGGACTTAAAAAGAGGTGTTTGGCAAGTAAAAAACTGTTTACGTCATC
TGTTACGTCTTCTGTTCACTTTATGTTTTACTCTCCACGCATCTTATCCTTTATAAGCTC
GCACAAATCTTAACCAAAACCAAAGCAAAACTTGAGAGTTTCTTACTAAGGTTGAATCAT
GGTTTCCGAAACAACCAAATCTTCTCCACTTCACTTTGTTCTCTTCCCTTTCATGGCTCA
AGGCCACATGATTCCCATGGTTGATATTGCAAGGCTCTTGGCTCAGCGTGGTGTGATCAT
AACAATTGTCACGACGCCTCACAATGCAGCGAGGTTCAAGAATGTCCTAAACCGTGCCAT
TGAGTCTGGCTTGCCCATCAACTTAGTGCAAGTCAAGTTTCCATATCTAGAAGCTGGTTT
GCAAGAAGGACAAGAGAATATCGATTCTCTTGACACAATGGAGCG
   
Sequence Name 18-K024507-022-371-C12C-HF01
GenBank Accession LN494144 [GenBank]
Sequencing primer gene specific primer, HF01: 5'-TTTAAAGAAAGGTATCATCCGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K024507-022-371-C12C-hf1
GTCAAGAGAATCGATATTCTCTTGTCCTTCTTGCAAACCAGCTTCTAGATATGGAAACTT
GACTTGCACTAAGTTGATGGGCAAGCCAGACTCAATGGCACGGTTTAGGACATTCTTGAA
CCTCGCTGCATTGTGAGGCGTCGTGACAATTGTTATGATCACACCACGCTGAGCCAAGAG
CCTTGCAATATCAACCATGGGAATCATGTGGCCTTGAGCCATGAAAGGGAAGAGAACAAA
GTGAAGTGGAGAAGATTTGGTTGTTTCGGAAACCATGATTCAACCTTAGTAAGAAACTCT
CAAGTTTTGCTTTGGTTTTGGTTAAGATTTGTGCGAGCTTATAAAGGATAAGATGCGTGG
AGAGTAAAACATAAAGTGAACAGAAGACGTAACAGATGACGTAAACAGTTTTTTACTTGC
CAAACACCTCTTTTTAAGTCCACAGATTGTGCTTGCTACTTAGTTTGTCCGTATGTGACC
ATCGTAGTGACCTTCCTTCTCAGGAAAAGTTTAGAAACATATCTTCATAAAGAAAACGAA
GATAATTCACATACACAAAACAACAACTTGGAGGGCTCACAATGATAAATTCAATTGAAA
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG

Last Updated on 06.12.2018 16:11