Sequences from confirmation amplicon


Confirmation sequences for line 497H12 and hit At2g37970 are shown.
Line ID 497H12
Locus At2g37970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15891016
Primers used for PCR GA68 - 8474, show primer details
   
Sequence Name 47-K101501-0022-497-H12C-8409
GenBank Accession LN506152 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K101501-0022-497-H12C-8409
CATTTACATTGAATATATCCTACTTCTCCTATGAGAATAGTTTTTGGTAAAACTTACATA
ATGGGAATGGTTTTTGGTAAAATCGCTGTAGAGACTCCTAAATACACGGTGACTAAATCC
GGTGACGGTTATGAGATCCGTGAATATCCACCAGCGGTTGCGGCGGAGGTTACATACGAT
GCGTCGGAGTTCAAAGGTGATAAAGACGGAGGCTTTCAGCTTTTGGCTAAGTACATAGGT
GTGTTTGGCAAACCGGAGAATGAGAAACCGGAGAAAATTGCTATGACTGCACCGGTGATC
ACTAAGGAAGGTGAGAAGATTGCGATGACTGCTCCGGTGATTACTAAGGAGAGTGAGAAG
ATTGAGATGACTTCTCCGGTTGTAACTAAGGAAGGTGGTGGAGAAGGAAGGAAGAAGTTG
GTGACGATGCAGTTTTTGTTGCCGTCGATGTATAAGAAGGCGGAGGAGGCACCACGTCCA
ACGGATGAGAGGGTTGTGATTAAGGAGGAAGGAGGGAGGAAGTATGGTGTGATTA
   
Sequence Name 47-K101505-0022-497-H12C-GA68
GenBank Accession LN506153 [GenBank]
Sequencing primer gene specific primer, GA68: 5'-ATCATGACCTCATTGGTCCTAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K101505-0022-497-H12C-GA68
CTAGCAGAACGAAATCTCCGGTGATCTTAAACCCATCTTTCTCAAGATGACTACTCAGCT
TCTTCACCTTCTCACTCACCACACTCTCCGACGCTATACCACTAAACTTAATCACACCAT
ACTTCCTCCCTCCTTCCTCCTTAATCACAACCCTCTCATCCGTTGGACGTGGTGCCTCCT
CCGCCTTCTTATACATCGACGGCAACAAAAACTGCATCGTCACCAACTTCTTCCTTCCTT
CTCCACCACCTTCCTTAGTTACAACCGGAGAAGTCATCTCAATCTTCTCACTCTCCTTAG
TAATCACCGGAGCAGTCATCGCAATCTTCTCACCTTCCTTAGTGATCACCGGTGCAGTCA
TAGCAATTTTCTCCGGTTTCTCATTCTCCGGTTTGCCAAACACACCTATGTACTTAGCCA
AAAGCTGAAAGCCTCCGTCTTTATCACCTTTGAACTCCGACGCATCGTANGTAACCTCCG
CCGNCACCGCTGGTGGATATTCACGGATCTCATAACCGTCACCGGATTTAGTCACCGTGT
ATTTAGGAATCTCTACNGCGATNTTACCAAAAACCATTCCCATTATGTAAGTTTTACCAA
AAACTATTCTCATA

Last Updated on 06.12.2018 16:11