Sequences from confirmation amplicon


Confirmation sequences for line 756F02 and hit At2g38100 are shown.
Line ID 756F02
Locus At2g38100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15949241
Primers used for PCR 08up - 8474, show primer details
   
Sequence Name 56-K117497-0022-756-F02C-08up
GenBank Accession LN509220 [GenBank]
Sequencing primer gene specific primer, 08up: 5'-ACCAACTAGAAGACGGTAGGAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K117497-0022-756-F02C-08up
AAATGTGGGGATTTTGTGTTCTTGTTACTTGCTGCTATTGCAATGCCTCAGATAAGTCCG
TGGTTTGTTAGGTTCACGATCCCCTCGGGCTGTGAGGTATTAGCAATGCTGATTTTTATT
TCCGGTGCGTGTTCATACAAACGGGTTAAACCAGGTGGGAGCCCTTTGACAACGGTTTTC
CGGGTCTTTATGGCTTCTGCTTCCAAAATGTCTTGTGCATACTCGAACAACTCAAGCCAG
CTCTATGAAAAAGCTGAGTGTGATCAAGATATTAAGCCCCACACAAGCAGCTTAAGGTTC
AATAAAAATTCACTTCATTAAGAGTTGTTAACCCACACTACTGTATAACACTCGTAACTC
TTGATTTTTTCAAACACAGGTATCTAGATAGAGCTGCAATGATACTCCAAACAGAGTCTT
TGGAACAACAGAGGAAAAACAGATGGAAGCTTTGTCGGGTGACAGAAGTGGATCCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCC
   
Sequence Name 24-K117496-0022-756-F02C-8409
GenBank Accession LN497164 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K117496-0022-756-F02C-8409
TCATTGAATATATCCTGGATCCACTTCTGTCACCCGACAAAGCTTCCATCTGTTTTTCCT
CTGTTGTTCCAAAGACTCTGTTTGGAGTATCATTGCAGCTCTATCTAGATACCTGTGTTT
GAAAAAATCAAGAGTTACGAGTGTTATACAGTAGTGTGGGTTAACAACTCTTAATGAAGT
GAATTTTTATTGAACCTTAAGCTGCTTGTGTGGGGCTTAATATCTTGATCACACTCAGCT
TTTTCATAGAGCTGGCTTGAGTTGTTCGAGTATGCACAAGACATTTTGGAAGCAGAAGCC
ATAAAGACCCGGAAAACCGTTGTCAAAGGGCTCCCACCTGGTTTAACCCGTTTGTATGAA
CACGCACCGGAAATAAAAATCAGCATTGCTAATACCTCACAGCCCGAGGGGATCGTGAAC
CTAACAAACCACGGACTTATCTGAGGCATTGCAATAGCAGCAAGTAACAAGAACACAAAA
TTCCCCACATTTCCAATGACAAAGCTAACGAGCTTGGCGGGGTTTCCCTTGTTCCTAC

Last Updated on 06.12.2018 16:11