Sequences from confirmation amplicon


Confirmation sequences for line 287E08 and hit At2g38185 are shown.
Line ID 287E08
Locus At2g38185 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16002809
Primers used for PCR 30qa - 8474, show primer details
   
Sequence Name 89-K115459-0022-287-E08C-30qa
GenBank Accession LN514976 [GenBank]
Sequencing primer gene specific primer, 30qa: 5'-GATGTTCCAAGAGATTGCTTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K115459-0022-287-E08C-30qa
ATGCGGACACGTAAGTTATTTTGTCCCGAAGGTCTTTAAGAATTGATAATATACAACTAG
ACATATCAAAATCACTTGATTGATCTAAACTTTGAAACGTTATGAATCTGCAATATGTGA
AGGATGCAAGAAGCTGATGGGAGTTGTCCAATATGTCGAAGGAAAATGAAGAAAGTGAAG
CGAATCTATACAGTGTAAGATGAATCAAATATAAATCTCTGACATAATGTTGAATCTATA
GAGCATTAATAAAAGTTATATTGGTATTGCCAAGGCTCATTTCTTTCTTTGCATAACTAT
TTCATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACAGGTTATT
   
Sequence Name 40-K115458-0022-287-E08C-8409
GenBank Accession LN503816 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K115458-0022-287-E08C-8409
ATTGAATGAATAGTTATGCAAAGAAAGAAATGAGCCTTGGCAATACCAATATAACTTTTA
TTAATGCTCTATAGATTCAACATTATGTCAGAGATTTATATTTGATTCATCTTACACTGT
ATAGATTCGCTTCACTTTCTTCATTTTCCTTCGACATATTGGACAACTCCCATCAGCTTC
TTGCATCCTTCACATATTGCAGATTCATAACGTTTCAAAGTTTAGATCAATCAAGTGATT
TTGATATGTCTAGTTGTATATTATCAATTCTTAAAGACCTTCGTGGACAAAATAACTTAC
GTTGTTCCGCATTCATAACAGGAGACGGAATGACCACAAGGGAGGAAAAAGCAATCTTNG
GGAACAT

Last Updated on 06.12.2018 16:11