Sequences from confirmation amplicon


Confirmation sequences for line 734G11 and hit At2g40815 are shown.
Line ID 734G11
Locus At2g40815 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17035618
Primers used for PCR BE12 - 8474, show primer details
   
Sequence Name 59-K028101-0022-734-G11C-BE12
GenBank Accession LN509889 [GenBank]
Sequencing primer gene specific primer, BE12: 5'-CTTGAAGGTAAGTCGATTCCTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K028101-0022-734-G11C-BE12
AGCTCCACTACTGCACCAGCTCCCACTCCTACAGCAAACCCTGGGCCAGTCCTAGCATAT
CCTCTTCCCGCCCCTCCTGGTACTCCTCCAGATGATGATGACGATGGAATGTTCGTAACA
TTCTCTGAATTAGGGATGTATGTAGGCATGTACCCTGAGTTCATAGGTGGTGGTGCTGGC
TGCATTGGTGGAGGATAGTAAGGTTGTTGCATTTGTGGGTTGGTGTTGTTCATTGGAGGG
TTAGGCATTGATGAGTCATTCACAGATTTGGGGATGTATGTAGGCATGTACCCTGAGTTC
ATAGGTGGTGGTGGTGGCTGCATCGGTGGAGGATAGTAAGGTTGTTGCATTTGTGGGTTG
GTGTTGGTCATTGGAGGGTTAGGCATTGATGAGTGGTGGTTATATGATGGGGGAA
   
Sequence Name 59-K028101-0022-734-G11C-8409
GenBank Accession LN509888 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K028101-0022-734-G11C-8409
CTGATCGTAGTTTATTTGCATTTTGTCTGCAATCAGATTATATGTAAATTGAACAACAAT
AGACGCAAATGTGAATGGGTTGTGTCAATTTGCAGAAAGAAACCAACTTTGGTGCTGCTT
TTGTTTCCTGTAACTCAATTCTTGAAGAACCTTAAATTTGGGTTTCCGAATATCCGATTT
CGAGATCTTAGCGTTTTTAAGATGGGTTTGTCTGAAGGAGAGCGCTCACCATCTTTCTCT
TCTTCTTTTGTGCAGGAGATTTTGGAGGAGTCATGCTCTCGAACAACTCAGATTACAACA
CTTCAGGACAGGATTACATGGCTGGTTCATCGCAGTATCCATTTGCTCCAATGGACCAGT
CAAACCCATTCTCAGTTCCCCCATCATATAACCACCACTCATCAATGCCTAACCCTCCAA
TGACCAACACCAACCCACAAATGCAACAACCTTACTATCCTCCACCGATGCAGCCACCAC
CACCACCTATGAACTCAGGGTACATGCCTACATACATCCCCAAATCTGTGAATGACTCAT
CAATGCCTAACCCTCCAATGAACAACACCAACCCACAAATGCAACAACCTTACTATCCTC
CACCAATGCAGCCAGCACCACCACCTATGAACTCAGGGTACATGCCTACATACATCCCTA
ATTCAGAGAATGTTACGAACATTCCATCGTCATCATCANTCTGGAGGAGTACCAGGAGGG
GCGGGAAGAGGATATGCTAGGACTGGCCCAGGGTTTGCTGTAGGAGTGGGAGCTGGTGCA
GTAGTTGGAGCTGGTGCAGCTTTATATGGAAGCGAGTTTATGTC

Last Updated on 06.12.2018 16:11