Sequences from confirmation amplicon


Confirmation sequences for line 957C09 and hit At2g40930 are shown.
Line ID 957C09
Locus At2g40930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17079723
Primers used for PCR 03ae - 8474, show primer details
   
Sequence Name 47-K106635-0022-957-C09C-8409
GenBank Accession LN506207 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K106635-0022-957-C09C-8409
CGGAATGAGCCTTTACATTGAATAATCCTGCCAAGGAACACTACCCTAATCAACTTCTAA
TGTAACTTCTGATGATATAAGATGTCTTATATATTCCAACGTTATTCCATTTATATGCAA
AATTAAGCCTATCAAGAATGTTTGGGACGAAAGCACAACCCCAAACATACAATACTGCAA
AAGATTTTACGTGCAACAGGGAAACACAAGTGAACCAATGATGTCTAAGCATACCTATAC
ACTTAAACCTACACACACACTTATGTATCCATCAGATTTATGAGTAGCAAAGAACTTCAA
GAGGAACTTACCACCATTCCAAGAGGGTTTTGCCAATTTATTTCTTGATGATAATCTTCT
TGAAAGTAACTAGCAAACTCAGGTGTATGAACCAGACACTGAATCGCACTATTCATGAAA
CAAGTATTCCCAAGATTTAGCAGCCCTGTCAAGCCAGCTGTGGATCCCCTAGTGGTGACG
CCAGTGCTACTGATTGTGTTA
   
Sequence Name 63-K106636-0022-957-C09C-03ae
GenBank Accession LN510999 [GenBank]
Sequencing primer gene specific primer, 03ae: 5'-ATAACACATACAGTAGCACTGGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K106636-0022-957-C09C-03ae
GGCTGCTAAATCTTGGGAATACTTGTTTCATGAATAGTGCGATTCAGTGTCTGGTTCATA
CACCTGAGTTTGCTAGTTACTTTCAAGAAGATTATCATCAAGAAATAAATTGGCAAAACC
CTCTTGGAATGGTGGTAAGTTCCTCTTGAAGTTCTTTGCTACTCATAAATCTGATGGATA
CATAAGTGTGTGTGTAGGTTTAAGTGTATAGGTATGCTTAGACATCATTGGTTCACTTGT
GTTTCCCTGTTGCACGTAAAATCTTTTGCAGTATTGTATGTTTGGGGTTGTGCTTTCGTC
CCAAACATTCTTGATAGGCTTAATTTTGCATATAAATGGAATAACGTTGGAATATATAAG
ACATCTTATATCATCAGAAGTTACATTAGAAGTTGATTAGGGTAGTGTTCCTTGGCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCA
Primers used for PCR 02bg - 8474, show primer details
   
Sequence Name 92-K118414-0022-957-C09C-02bg
GenBank Accession LN515213 [GenBank]
Sequencing primer gene specific primer, 02bg: 5'-TATAACCAAATGCAAGCCTAATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K118414-0022-957-C09C-02bg
AACAGAGAAGCGTTGGATTTTCTAAAAGATATACAGCTGACCTGACAAACATCAACTATT
ATTGAGTCATTACGAGCAATATGATTTTTCCAGAACTCATCAGCAACTTCTTCATCAGGG
CGCCCATCTGCGTCTCGAGAATTTATATATGGTTTGTGTTTAACACGATTTAGGTCCTCG
TGAAGACCATCCAGCAGAAACGCTAAAAGCTCCTGCAAAATGAAAAACTCGAATTCCTTG
AGCTACAATGTAAATTCAGGTTAAAGAAAATATCTGGCTAGTCTCACAAACCTGCGAATC
ATGTTGATTGTACCCACTAAATTGAGGAGCAAAGCGAGCAAGTTTAGCTTTGAATGGACG
AGGTGCAATTGGTGTCCGCCCAGGAGCCCATAACTTCCTGAGCAAGTCACCAAATGCGAG
AGCTAATTCCCCCTGTGCAAAATGATTTCAATACAGTCAAGAAATGATCTGAAATCAACT
ACTCAATATCTCAGAAAGAAACAATGCTTGTGATCTACGCGAGAAAATAACACTGATGAG
TAATACAAGTACATTTACATGGATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 44-K118413-0022-957-C09C-8409
GenBank Accession LN505338 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K118413-0022-957-C09C-8409
TTGATCCATGTAAATGTACTTGTATTACTCATCAGTGTTATTTTCTCGCGTAGATCACAA
GCATTGTTTCTTTCTGAGATATTGAGTAGTTGATTTCAGATCATTTCTTGACTGTATTGA
AATCATTTTGCACAGGGGGAATTAGCTCTCGCATTTGGTGACTTGCTCAGGAAGTTATGG
GCTCCTGGGCGGACACCAATTGCACCTCGTCCATTCAAAGCTAAACTTGCTCGCTTTGCT
CCTCAATTTAGTGGGTACAATCAACATGATTCGCAGGTTTGTGAGACTAGCCAGATATTT
TCTTTAACCTGAATTTACATTGTAGCTCAAGGAATTCGAGTTTTTCATTTTGCAGGAGCT
TTTAGCGTTTCTGCTGGATGGTCTTCACGAGGACCTAAATCGTGTTAAACACAAACCATA
TATAAATTCTCGAGACGCAGATGGGCGCCCTGATGAAGAAGTTGCTGATGAGTTCTGGAA
AAATCATATTGCTCGTAATGACTCAATAATAGTTGATGTTTGTCAGGTCAGCTGTATATC
TTTTAGAAAATCCAACGCTTCTCTGTTTATCAACTCATGGCATTGAAACTGATACTAGTA
TTGTGGATTAGGCTTGCATTTGGGTTAT

Last Updated on 06.12.2018 16:11